Imperial/Infector Detector/Implementation
From 2007.igem.org
(Difference between revisions)
Line 46: | Line 46: | ||
|width="40%"| Not Required | |width="40%"| Not Required | ||
- | |width="40%"| LuxR protein ([[Imperial/Wet_Lab/Protocols/Prot1.5|Protocol]] and [ | + | |width="40%"| LuxR protein ([[Imperial/Wet_Lab/Protocols/Prot1.5|Protocol]] and [https://2007.igem.org/Imperial/Wet_Lab/Results/Res1.5 Results]) |
|- | |- | ||
|width="20%" style="background-color:#ddeeff" align="center" | '''Stage 5: Quality Control''' <br /> <br>[[Image:Icegems-Devcycle-downarrow.png|30px]] | |width="20%" style="background-color:#ddeeff" align="center" | '''Stage 5: Quality Control''' <br /> <br>[[Image:Icegems-Devcycle-downarrow.png|30px]] | ||
|width="40%"| | |width="40%"| | ||
- | * '''Testing ''in vivo''''': Passed ([[Imperial/Wet_Lab/Protocols/ID1.1|Protocol]] and [ | + | * '''Testing ''in vivo''''': Passed ([[Imperial/Wet_Lab/Protocols/ID1.1|Protocol]] and [https://2007.igem.org/Imperial/Wet_Lab/Results/ID1.1 Results]) |
- | * '''Sequencing of Construct''': | + | * '''Sequencing of Construct''': Completed |
|width="40%"| | |width="40%"| | ||
- | * '''Testing ''in vitro''''': Passed ([[Imperial/Wet_Lab/Protocols/Prot1.7|Protocol]] and [ | + | * '''Testing ''in vitro''''': Passed ([[Imperial/Wet_Lab/Protocols/Prot1.7|Protocol]] and [https://2007.igem.org/Imperial/Wet_Lab/Results/ID1.7 Results]) |
- | * '''Sequencing of Construct''': | + | * '''Sequencing of Construct''': Completed |
|- align="center" | |- align="center" | ||
|width="20%" style="background-color:#ddeeff"| '''Stage 6: Final Implementation Status''' | |width="20%" style="background-color:#ddeeff"| '''Stage 6: Final Implementation Status''' | ||
Line 80: | Line 80: | ||
|width="20%" style="background-color:#ddeeff" align="center" | '''Stage 3: Quality Control''' <br /> <br> [[Image:Icegems-Devcycle-downarrow.png|30px]] | |width="20%" style="background-color:#ddeeff" align="center" | '''Stage 3: Quality Control''' <br /> <br> [[Image:Icegems-Devcycle-downarrow.png|30px]] | ||
|width="40%"| | |width="40%"| | ||
- | * '''Testing ''in vitro''''': | + | * '''Testing ''in vitro''''': Failed ([[Protocol and Results) |
|width="40%"| | |width="40%"| | ||
- | * '''Testing ''in vitro''''': Passed ([[Imperial/Wet_Lab/Protocols/ID1.2|Protocol]] and [ | + | * '''Testing ''in vitro''''': Passed ([[Imperial/Wet_Lab/Protocols/ID1.2|Protocol]] and [https://2007.igem.org/Imperial/Wet_Lab/Results/ID1.2 Results]) |
|- align="center" | |- align="center" | ||
|width="20%" style="background-color:#ddeeff"| '''Stage 4: Final Implementation Status''' | |width="20%" style="background-color:#ddeeff"| '''Stage 4: Final Implementation Status''' |
Revision as of 01:28, 26 October 2007
Infector Detector: Implementation
| ||
Construct Design | ||
Stage 1: Availability | Found as [http://partsregistry.org/Part:BBa_T9002 BBa_T9002] in Registry. | Found as [http://partsregistry.org/Part:BBa_J37032 BBa_J37032] in Registry. |
Stage 2: Cloning / Amplification | ||
Stage 3: Ligation | Not Required | Not Required |
Stage 4: Purification of Activator Proteins | Not Required | LuxR protein (Protocol and Results) |
Stage 5: Quality Control | ||
Stage 6: Final Implementation Status | Ready for Testing | Aborted. LuxR protein not functional. |
| ||
Chassis Design | ||
Stage 1: Availability | Possibility of making Homemade S30 Cell Extract. | Commercially available S30 Cell Extract purchasable from [http://www.promega.com/search/Default.aspx?query=s30+extract Promega]. |
Stage 2: Preparation | Preparation: Done (Protocol) | Preparation: Done (Protocol) |
Stage 3: Quality Control |
| |
Stage 4: Final Implementation Status | Aborted. Expression capacity of chassis was low. | Ready for Testing |