Revision as of 19:25, 26 October 2007

Infector Detector: Implementation

DNA Construct Implementation
Construct Design
Stage 1: Availability	Found as [http://partsregistry.org/Part:BBa_T9002 BBa_T9002] in Registry.	Found as [http://partsregistry.org/Part:BBa_J37032 BBa_J37032] in Registry.
Stage 2: Cloning / Amplification	BBa_T9002 - Lane 18 (1945bp)	BBa_J37032 - Lane R25 (938bp + 2079bp vector)
Stage 3: Ligation	Not Required	Not Required
Stage 4: Purification of Activator Proteins	Not Required	Purification of LuxR protein (Protocol and Results)
Stage 5: Quality Control	Testing in vivo: Passed (Protocol and Results) Sequencing of Construct: Partially Sequenced	Testing in vitro: Failed (Protocol and Results) Sequencing of Construct: Completed
Stage 6: Final Implementation Status	Ready for Testing	Aborted. LuxR protein not functional.

Chassis Construct Implementation
Chassis Design
Stage 1: Availability	Possibility of making Homemade S30 Cell Extract.	Commercially available S30 Cell Extract purchasable from [http://www.promega.com/search/Default.aspx?query=s30+extract Promega].
Stage 2: Preparation	Preparation: Done (Protocol)	Preparation: Done (Protocol)
Stage 3: Quality Control	Testing in vitro: Failed (Protocol and Results)	Testing in vitro: Passed (Protocol and Results)
Stage 4: Final Implementation Status	Aborted. Expression capacity of chassis was low.	Ready for Testing

<< Modelling | Implementation | Testing/Validation >>

@@ Line 51: / Line 51: @@
 |width="40%"| [[Image:IC_2007_Plux_in_vivo.PNG|center|350px]]<br />
 * '''Testing ''in vivo''''': Passed ([[Imperial/Wet_Lab/Protocols/ID1.1|Protocol]] and [https://2007.igem.org/Imperial/Wet_Lab/Results/ID1.1 Results])
-* '''Sequencing of Construct''': Completed
+* '''Sequencing of Construct''': Partially Sequenced
 |width="40%"| [[Image:IC_2007_LuxR_testing_1.PNG|center|350px]]<br />
 * '''Testing ''in vitro''''': Failed ([[Imperial/Wet_Lab/Protocols/Prot1.7|Protocol]] and [https://2007.igem.org/Imperial/Wet_Lab/Results/ID1.7 Results])
@@ Line 80: / Line 80: @@
 |width="20%" style="background-color:#ddeeff" align="center" | '''Stage 3: Quality Control''' <br /> <br> [[Image:Icegems-Devcycle-downarrow.png|30px]]
 |width="40%"|
-* '''Testing ''in vitro''''': Failed ([[Protocol and Results)
+* '''Testing ''in vitro''''': Failed ([[Imperial/Wet_Lab/Protocols/CE1.5|Protocol]] and [[Imperial/Wet_Lab/Results/Chas1.1 |Results]])
 |width="40%"|
 * '''Testing ''in vitro''''': Passed ([[Imperial/Wet_Lab/Protocols/ID1.2|Protocol]] and [https://2007.igem.org/Imperial/Wet_Lab/Results/ID1.2 Results])