Latest revision as of 02:33, 27 October 2007

Infector Detector: Implementation

DNA Construct Implementation
Construct Design
Stage 1: Availability	Found as [http://partsregistry.org/Part:BBa_T9002 BBa_T9002] in Registry.	Found as [http://partsregistry.org/Part:BBa_J37032 BBa_J37032] in Registry.
Stage 2: Cloning / Amplification	BBa_T9002 - Lane 18 (1945bp)	BBa_J37032 - Lane R25 (938bp + 2079bp vector)
Stage 3: Ligation	Not Required	Not Required
Stage 4: Purification of Activator Proteins	Not Required	Purification of LuxR protein (Protocol and Results)
Stage 5: Quality Control	Testing in vivo: Passed (Protocol and Results) Sequencing of Construct: Partially Sequenced	Testing in vitro: Failed (Protocol and Results) Sequencing of Construct: Completed
Stage 6: Final Implementation Status	Ready for Testing	Aborted. LuxR protein not functional.

Chassis Construct Implementation
Chassis Design
Stage 1: Availability	Possibility of making Homemade S30 Cell Extract.	Commercially available S30 Cell Extract purchasable from [http://www.promega.com/search/Default.aspx?query=s30+extract Promega].
Stage 2: Preparation	Preparation: Done (Protocol)	Preparation: Done (Protocol)
Stage 3: Quality Control	Testing in vitro: Failed (Protocol and Results)	Testing in vitro: Passed (Protocol and Results)
Stage 4: Final Implementation Status	Aborted. Expression capacity of chassis was low.	Ready for Testing

<< Modelling | Implementation | Testing/Validation >>

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