Template:BerkiGEM2007 AustinConstructionFiles
From 2007.igem.org
pBca1101-I716051 (built): Kan cassette RBS pool
pBca1101-I716052(built): Cmr cassette RBS pool
9145-I716053(built): alpha HbA in 9145(biobricks 2.0 format)
9145-I716054(built): beta HbA in 9145(biobricks 2.0 format)
9145-I716055(built): AHSP in 9145(biobricks 2.0 format)
9145-I716056(built): -kan-RBS library-alpha HbA-
9145-I716057(built): -CmR-RBS library-beta HbA-
9145-I716058(built): 1X mutant HbA alpha (L29F) AKA: I716053m29
9145-I716059(built): 1X mutant HbA beta (Asn108Lys) AKA: I716054m108
9145-I716060(built): beta-alpha cassette (with RBS libraries)
9145-I716061(built): pTRC-rbs-beta-rbs-alpha
9145-I716062(built): 2X mutant HbA alpha (L29F/V96W) AKA: I716053m29m96
9145-I716063(built): 2X mutant HbA beta (Asn108Lys/Lys82Asp) AKA: I716054m108m82
1103-I716064: -pCon-(no scar site)-rbs-beta-rbs-alpha
9145-I716065(built): 2X mutant -Kan-rbs-alpha Hba (L29F and V96W)
9145-I716066(built): 2X mutant -Cmr-beta Hba (Asn108Lys and Lys82Asp)
9145-I716067(built): 2X mutants -rbs library-beta-rbs library-alpha- (with RBS libraries)
9145-I716068: 2X mutant Di-Alpha fusion, biobricked
pTrc-I716069(built): 2X mutant -pTRC-rbs-beta-rbs-alpha L29F/V96W and Asn108Lys/Lys82Asp
9145-I716070(built): alpha HbA-TT
9145-I716071: pTrc-(NO SCAR)-rbs lib-beta-rbs lib-alpha
9145-I716072: 1X mutant beta Hba (Lys82Asp "Providence")
9145-I716073(built): unmutated Di-alpha fusion, biobricked
9145-I716074: pTrc-rbs lib-(NO SCAR)-beta 1X mutant, providence (Lys82Asp) - rbs lib - unmutated di-alpha- (Best Yield)
9145-I716075: pTrc-rbs lib-(NO SCAR)-beta 2X mutant, providence (Lys82Asp) and Presbyterian (Asn108Lys)-rbs lib - unmutated di alpha - (Best all around)
9145-I716076: pTrc-rbs lib-(NO SCAR)-1X mutant beta, presbyterian (Asn108Lys)-rbs lib-2X mutant alpha (L29F and V96W) (Second best all around)
9145-I716077: Unmutated Di-Alpha fusion - TT
9145-I716078: 2X mutant alpha - TT
9145-I716079: 2X mutant Di-alpha fusion -TT
pTRC-I716080: pTRC-rbs-beta
pTRC-I716081: pTRC-rbs-1X mutant beta (providence Lys82Asp)
pTRC-I716082: pTRC-rbs-2X mutant beta (providence/Presbyterian Lys82Asp/Asn108Lys)
9145-I716083:
9145-I716084:
9145-I716085:
9145-I716086:
9145-I716087:
9145-I716088:
9145-I716089:
9145-I716090:
9145-I716091:
9145-I716092:
9145-I716093:
9145-I716094:
9145-I716095:
9145-I716096:
9145-I716097:
9145-I716098:
9145-I716099:
