Imperial/Infector Detector/Implementation

From 2007.igem.org

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== Chassis Status ==
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|width="40%" style="background-color:#eeffee"| <center>'''Chassis'''</center>
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|colspan="3" style="background-color:#eeeeff"| <center>'''Chassis Construct Implementation'''</center>
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|width="--" style="background-color:#eeffee"| <center>'''Availability Status'''</center>
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|width="20%" style="background-color:#ddeeff"| '''Chassis Design'''
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|width="40%"| <center>Promega ''E. coli'' S30 Cell Extract </center>
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|colspan="2" align="center" |[[Image:IC07_CFS_components.png|300px]]
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|width="--"| <center> Purchased </center>
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|width="20%" style="background-color:#ddeeff"| '''Stage 1: Availability'''
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|width="40%"| Possibility of making Homemade S30 Cell Extract.
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|width="40%"| Commercially available S30 Cell Extract purchasable from [http://www.promega.com/search/Default.aspx?query=s30+extract Promega].
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|width="20%" style="background-color:#ddeeff"| '''Stage 2: Preparation'''
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|width="40%"| '''Preparation''': Done ([[Imperial/Wet_Lab/Protocols/CE1.1|Protocol]])
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|width="40%"| '''Preparation''': Done ([[Imperial/Wet_Lab/Protocols/CE1.3|Protocol]])
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|width="20%" style="background-color:#ddeeff" align="center" | '''Stage 3: Quality Control'''
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* '''Testing ''in vitro''''': Done (Protocol and Results)
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* '''Testing ''in vitro''''': Done ([[Imperial/Wet_Lab/Protocols/ID1.2|Protocol]] and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/ID1.2 Results])
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|width="20%" style="background-color:#ddeeff"| '''Stage 6: Final Implementation Status'''
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|width="40%" style="background-color:#ddb99e"| Aborted. <br /> Expression capacity of chassis was low.
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|width="40%" style="background-color:#b8efb0"| Ready for Testing
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(Link to Commercial Preparation Protocols)
 
<center>  [https://2007.igem.org/Imperial/Infector_Detector/Modelling << Modelling] | Implementation | [https://2007.igem.org/Imperial/Infector_Detector/Testing Testing/Validation >>]
<center>  [https://2007.igem.org/Imperial/Infector_Detector/Modelling << Modelling] | Implementation | [https://2007.igem.org/Imperial/Infector_Detector/Testing Testing/Validation >>]
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Revision as of 12:08, 24 October 2007


Infector Detector: Implementation

DNA Construct Implementation
Construct Design Imperial ID Design ID1.png Imperial ID Design ID2.png
Stage 1: Availability Found as BBa_T9002 in Registry. Found as BBa_J37032 in Registry.
Stage 2: Cloning / Amplification
BBa_T9002 - Lane 18 (1945bp)
BBa_J37032 - Lane R25 (938bp + 2079bp vector)
Stage 3: Ligation Not Required Not Required
Stage 4: Purification of Activator Proteins Not Required LuxR protein (Protocol and Results)
Stage 5: Quality Control
  • Testing in vivo: Done (Protocol and Results)
  • Sequencing of Construct: Pending
  • Testing in vitro: Done (Protocol and Results)
  • Sequencing of Construct: Pending
Stage 6: Final Implementation Status Ready for Testing Aborted.
LuxR protein not functional.



Chassis Construct Implementation
Chassis Design IC07 CFS components.png
Stage 1: Availability Possibility of making Homemade S30 Cell Extract. Commercially available S30 Cell Extract purchasable from Promega.
Stage 2: Preparation Preparation: Done (Protocol) Preparation: Done (Protocol)
Stage 3: Quality Control
  • Testing in vitro: Done (Protocol and Results)
Stage 6: Final Implementation Status Aborted.
Expression capacity of chassis was low. 		Ready for Testing



<< Modelling | Implementation | Testing/Validation >>
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