Revision as of 14:12, 24 October 2007

Infector Detector: Implementation

DNA Construct Implementation
Construct Design	Concept 1	Concept 2
Stage 1: Availability	Found as BBa_T9002 in Registry.	Found as BBa_J37032 in Registry.
Stage 2: Cloning / Amplification	BBa_T9002 - Lane 18 (1945bp)	BBa_J37032 - Lane R25 (938bp + 2079bp vector)
Stage 3: Ligation	Not Required	Not Required
Stage 4: Purification of Activator Proteins	Not Required	LuxR protein (Protocol and Results)
Stage 5: Quality Control	Testing in vivo: Done (Protocol and Results) Sequencing of Construct: Pending	Testing in vitro: Done (Protocol and Results) Sequencing of Construct: Pending
Stage 6: Final Implementation Status	Ready for Testing	Aborted. LuxR protein not functional.

Chassis Construct Implementation
Chassis Design
Stage 1: Availability	Possibility of making Homemade S30 Cell Extract.	Commercially available S30 Cell Extract purchasable from Promega.
Stage 2: Preparation	Preparation: Done (Protocol)	Preparation: Done (Protocol)
Stage 3: Quality Control	Testing in vitro: Done (Protocol and Results)	Testing in vitro: Done (Protocol and Results)
Stage 6: Final Implementation Status	Aborted. Expression capacity of chassis was low.	Ready for Testing

<< Modelling | Implementation | Testing/Validation >>

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 |- align="center"
 |wifth="20%" style="background-color:#ddeeff"| '''Construct Design'''
-|width="40%"| [[Image:Imperial ID Design ID1.png]]
+|width="40%" align="left" | '''Concept 1''' <br />[[Image:Imperial ID Design ID1.png|center]]
-|width="40%"| [[Image:Imperial_ID_Design_ID2.png]]
+|width="40%" align="left" | '''Concept 2''' <br />[[Image:Imperial_ID_Design_ID2.png|center]]
 |- align="center"
 |width="20%" style="background-color:#ddeeff"| '''Stage 1: Availability'''