Template:BerkiGEM2007 AustinConstructionFiles
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| - | [[BerkiGEM2007-AustinConstructionFile1 | I716051: Kan cassette RBS pool]]<br> | + | '''1128 RBS Libraries: '''<br> |
| - | [[BerkiGEM2007- | + | [[BerkiGEM2007-AustinConstructionFile1 | pBca1101-I716051 (built): Kan cassette RBS pool]]<br> |
| - | [[BerkiGEM2007- | + | [[BerkiGEM2007-AustinConstructionFile2 | pBca1101-I716052(built): Cmr cassette RBS pool]]<br> |
| - | [[BerkiGEM2007- | + | |
| - | [[BerkiGEM2007- | + | '''Unmutated basic parts: '''<br> |
| + | [[BerkiGEM2007-AustinConstructionFile3 | 9145-I716053(built): alpha HbA in 9145(biobricks 2.0 format)]]<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile4 | 9145-I716054(built): beta HbA in 9145(biobricks 2.0 format)]]<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile5 | 9145-I716055(built): AHSP in 9145(biobricks 2.0 format)]]<br> | ||
| + | |||
| + | '''Cassette Construction Using 1128 RBS Library: '''<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile6 | 9145-I716056(built): -kan-RBS library-alpha HbA- ]]<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile7 | 9145-I716057(built): -CmR-RBS library-beta HbA-]]<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile10 | 9145-I716060(built): beta-alpha cassette (with RBS libraries)]]<br> | ||
| + | |||
| + | [[BerkiGEM2007-AustinConstructionFile15 | 9145-I716065(built): 2X mutant -Kan-rbs-alpha Hba (L29F and V96W)]]<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile16 | 9145-I716066(built): 2X mutant -Cmr-beta Hba (Asn108Lys and Lys82Asp)]]<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile17 | 9145-I716067(built): 2X mutants -rbs library-beta-rbs library-alpha- (with RBS libraries)]]<br> | ||
| + | |||
| + | [[BerkiGEM2007-AustinConstructionFile11 | 9145-I716061(built): pTRC-rbs-beta-rbs-alpha]]<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile19 | pTrc-I716069(built): 2X mutant -pTRC-rbs-2x beta-rbs-2x alpha L29F/V96W and Asn108Lys/Lys82Asp]]<br> | ||
| + | |||
| + | '''HbA Subunit Mutant Variants: '''<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile8 | 9145-I716058(built): 1X mutant HbA alpha (L29F) AKA: I716053m29]]<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile9 | 9145-I716059(built): 1X mutant HbA beta (Asn108Lys "Presbyterian") AKA: I716054m108]]<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile12 | 9145-I716062(built): 2X mutant HbA alpha (L29F/V96W) AKA: I716053m29m96]]<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile13 | 9145-I716063(built): 2X mutant HbA beta (Asn108Lys/Lys82Asp "Providence/Presbyterian") AKA: I716054m108m82]]<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile22 | 9145-I716072(built): 1X mutant beta Hba (Lys82Asp "Providence")]]<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile23 | 9145-I716073(built): unmutated Di-alpha fusion, biobricked]]<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile18 | 9145-I716068(built): 2X mutant Di-Alpha fusion, biobricked]]<br> | ||
| + | |||
| + | '''No Scar Rbs-Part RBS library'''<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile14 | 1103-I716064: -pCon-(no scar site)-rbs-beta-rbs-alpha]]<br> | ||
| + | |||
| + | [[BerkiGEM2007-AustinConstructionFile20 | 9145-I716070(built): alpha HbA-TT]]<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile27 | 9145-I716077(built): Unmutated Di-Alpha fusion - TT]]<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile28 | 9145-I716078(built): 2X mutant alpha - TT]]<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile29 | 9145-I716079(built): 2X mutant Di-alpha fusion -TT]]<br> | ||
| + | |||
| + | [[BerkiGEM2007-AustinConstructionFile30 | 9145-I716080(built): -rbs-(no scar)-beta]]<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile31 | 9145-I716081(built): -rbs-(no scar)-1X mutant beta (providence Lys82Asp)]]<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile32 | 9145--I716082(built): -rbs-(no scar)-2X mutant beta (providence/Presbyterian Lys82Asp/Asn108Lys)]]<br> | ||
| + | |||
| + | [[BerkiGEM2007-AustinConstructionFile21 | 9145-I716071(built): -rbs lib-(NO SCAR)-beta-rbs lib-(no scar)-alpha-TT]]<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile24 | 9145-I716074(built): -rbs lib-(NO SCAR)-beta 1X mutant, providence (Lys82Asp) - rbs lib-(no scar) - unmutated di-alpha-TT- (Best Yield)]]<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile25 | 9145-I716075(built): -rbs lib-(NO SCAR)-beta 2X mutant, providence (Lys82Asp) and Presbyterian (Asn108Lys)-rbs lib-(no scar) - unmutated di alpha - TT- (Best all around)]]<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile26 | 9145-I716076(built): -rbs lib-(NO SCAR)-1X mutant beta, presbyterian (Asn108Lys)-rbs lib-(no scar)-2X mutant alpha (L29F and V96W) - TT (Second best all around)]]<br> | ||
| + | |||
| + | [[BerkiGEM2007-AustinConstructionFile33 | 9145-I716083(built): pT7105-rbs lib-(NO SCAR)-beta-rbs lib-(no scar)-alpha-TT]]<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile34 | 9145-I716084(built): pT7105-rbs lib-(NO SCAR)-beta 1X mutant, providence (Lys82Asp) - rbs lib-(no scar) - unmutated di-alpha-TT- (Best Yield)]]<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile35 | 9145-I716085(built): pT7105-rbs lib-(NO SCAR)-beta 2X mutant, providence (Lys82Asp) and Presbyterian (Asn108Lys)-rbs lib-(no scar) - unmutated di alpha - TT- (Best all around)]]<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile36 | 9145-I716086(built): pT7105-rbs lib-(NO SCAR)-1X mutant beta, presbyterian (Asn108Lys)-rbs lib-(no scar)-2X mutant alpha (L29F and V96W) - TT (Second best all around)]]<br> | ||
| + | |||
| + | '''petDUET stuffs beta insertion'''<br> | ||
| + | |||
| + | [[BerkiGEM2007-AustinConstructionFile37 | 9145-I716087(built): (unmodified beta)insertion]]<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile38 | 9145-I716088(built): (Beta providence) insertion]]<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile39 | 9145-I716089(built): (Beta providence / Presbyterian) insertion]]<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile40 | 9145-I716090(built): (Beta presbyterian) insertion]]<br> | ||
| + | |||
| + | '''petDUET stuffs alpha and beta insertion'''<br> | ||
| + | |||
| + | [[BerkiGEM2007-AustinConstructionFile41 | 9145-I716091(built): unmodified beta + unmodified alpha: "Unmodified" in petDUET]]<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile42 | 9145-I716092: Beta providence + unmodified dialpha fusion: "Best Yield" in petDUET]]<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile43 | 9145-I716093(built): Beta providence / Presbyterian + unmodified dialpha fusion : "Best All Around" in petDUET]]<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile44 | 9145-I716094(built): Beta presbyterian + 2X mutant alpha L29F/V96W: "Second Best All Around" in petDUET]]<br> | ||
| + | |||
| + | '''(Basically Biobricked)Hemoglobin cassette + hemA/hemB: '''<br> | ||
| + | |||
| + | [[BerkiGEM2007-AustinConstructionFile45 | 9145-I716095 (built): "Unmodified" (I716091) + hemA/hemB cassette]]<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile46 | 9145-I716096 (built): "Best Yield" (I716092) + hemA/hemB cassette]]<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile47 | 9145-I716097 (built): "Best All Around" (I716093) + hemA/hemB cassette]]<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile48 | 9145-I716098: "Second Best All Around" (I716094) + hemA/hemB cassette]]<br> | ||
| + | |||
| + | '''(Basically Biobricked)Hemoglobin cassette + Cytochrome B5/B5 reductase cassette + hemA/hemB cassette: '''<br> | ||
| + | |||
| + | [[BerkiGEM2007-AustinConstructionFile49 | 9145-I716099(built): "Unmodified" (I716091) + cytochrome b5/b5reductase cassette + hemA/hemB cassette]]<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile50 | 9145-I716100: "Best Yield" (I716092) + cytochrome b5/b5 reductase cassette + hemA/hemB cassette]]<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile51 | 9145-I716351(built): "Best All Around" (I716093) + cytochrome b5/b5 reductase cassette + hemA/hemB cassette]]<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile52 | 9145-I716352: "Second Best All Around" (I716094) + cytochrome b5/b5reductase cassette + hemA/hemB cassette]]<br> | ||
| + | |||
| + | <br>'''(Basically Biobricked)Hemoglobin cassette + cytochrome b5/b5 reductase + hemA/hemB + sodC/katG cassette: '''<br> | ||
| + | |||
| + | [[BerkiGEM2007-AustinConstructionFile58 | 9145-I716358: "Unmodified" (I716091) + cytochrome b5/b5reductase cassette + hemA/hemB cassette + sodC/KatG]]<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile59 | 9145-I716359: "Best Yield" (I716092) + cytochrome b5/b5 reductase cassette + hemA/hemB cassette + sodC/katG]]<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile60 | 9145-I716360: "Best All Around" (I716093) + cytochrome b5/b5 reductase cassette + hemA/hemB cassette + sodC/KatG]]<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile61 | 9145-I716361: "Second Best All Around" (I716094) + cytochrome b5/b5reductase cassette + hemA/hemB cassette + sodC/KatG]]<br> | ||
| + | |||
| + | |||
| + | '''Detox constructs'''<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile53 | 9145-I716353: AdhE in petDUET]]<br> | ||
| + | <br> | ||
| + | '''Biobricked petDUET Hemoglobin Cassettes (XhoI/EcoRI/BglII sites removed)'''<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile54 | 9145-I716354: "Unmodified" (I716091)]]<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile55 | 9145-I716355: "Best Yield" (I716092)]]<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile56 | 9145-I716356: "Best All Around" (I716093)]]<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile57 | 9145-I716357: "Second Best All Around" (I716094)]]<br> | ||
| + | |||
| + | '''Different T7's with Hem Genes'''<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile66 | 9145-I716366: T7 Clone A with Hem genes A + B]]<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile67 | 9145-I716367: T7 Clone C with Hem genes A + B]]<br> | ||
| + | |||
| + | '''Biobricked petDUET Hemoglobin Cassettes + sodC/katG (Line discontinued)'''<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile62 | 9145-I716362: Unmodified]]<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile63 | 9145-I716363: "Best Yield" (Mutant Line Discontinuted)]]<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile64 | 9145-I716364: Best All Around]]<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile65 | 9145-I716365: "Second Best All Around" (Mutant Line Discontinuted)]]<br> | ||
| + | |||
| + | '''Biobricked petDUET Hemoglobin + SodC/KatG + Cytochrome b5/b5 reductase (Line discontinued)'''<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile68 | 9145-I716368: Unmodified cassette with antioxidants + cytochromes]]<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile69 | 9145-I716369: Best all around cassette with antioxidants + cytochromes]]<br> | ||
| + | |||
| + | '''Biobricked petDUET Hemoglobin + sodC/katG + Cytochromes + AHSP(Line discontinued)'''<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile70 | 9145-I716370: Unmodified cassette]]<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile71 | 9145-I716371: Best all around cassette]]<br> | ||
| + | |||
| + | '''Biobricked petDUET Hemoglobin + sodC/katG + Cytochromes + AHSP + metAP(Line discontinued)'''<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile72 | 9145-I716372: Unmodified cassette]]<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile73 | 9145-I716373: Best all around cassette]]<br> | ||
| + | |||
| + | '''Biobricked petDUET Hemoglobin Cassettes + heme'''<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile74 | 9145-I716374: Unmodified cassette]]<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile75 | 9145-I716375: Best all around cassette]]<br> | ||
| + | |||
| + | '''Biobricked petDUET Hemoglobin Cassettes + heme + Cytochromes'''<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile76 | 9145-I716376: Unmodified cassette]]<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile77 | 9145-I716377: Best all around cassette]]<br> | ||
| + | |||
| + | '''Biobricked petDUET Hemoglobin Cassettes + heme + AHSP'''<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile78 | 9145-I716378: Unmodified cassette]]<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile79 | 9145-I716379: Best all around cassette]]<br> | ||
| + | |||
| + | '''Biobricked petDUET Hemoglobin Cassettes + heme + sodC/katG'''<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile80 | 9145-I716380: Unmodified cassette]]<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile81 | 9145-I716381: Best all around cassette]]<br> | ||
| + | |||
| + | '''Biobricked petDUET Hemoglobin + heme + cytochromes + sodC/katG'''<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile83 | 9145-I716383: Unmodified]]<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile84 | 9145-I716384: Best all around]]<br> | ||
| + | |||
| + | '''Biobricked petDUET Hemoglobin + heme + cytochromes + sodC/katG + AHSP'''<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile85 | 9145-I716385: Unmodified]]<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile86 | 9145-I716386: Best all around]]<br> | ||
| + | |||
| + | '''Biobricked petDUET Hemoglobin + heme + cytochromes + sodC/katG + AHSP + metAP'''<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile87 | 9145-I716386: Unmodified Final Construct]]<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile88 | 9145-I716387: Best all around Final Construct]]<br> | ||
| + | |||
| + | '''Biobricked petDUET Hemoglobin + heme + sodC/katG + AHSP + metAP'''<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile89 | 9145-I716388: Unmodified]]<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile90 | 9145-I716389: Best all around]]<br> | ||
| + | |||
| + | '''HemeABCD in pBACb'''<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile82 | 9145-I716382: pBACb with hemABCD]]<br> | ||
| + | |||
| + | '''HemeABCD-TT in 9145'''<br> | ||
| + | [[BerkiGEM2007-AustinConstructionFile91 | 9145-I716390: HemABCD-TT in 9145]]<br> | ||
| + | |||
| + | |||
| + | '''Unused Numbers'''<br> | ||
| + | |||
| + | |||
| + | [[BerkiGEM2007-AustinConstructionFile92 | 9145-I716391: ]]<br> | ||
Latest revision as of 00:01, 16 October 2007
1128 RBS Libraries:
pBca1101-I716051 (built): Kan cassette RBS pool
pBca1101-I716052(built): Cmr cassette RBS pool
Unmutated basic parts:
9145-I716053(built): alpha HbA in 9145(biobricks 2.0 format)
9145-I716054(built): beta HbA in 9145(biobricks 2.0 format)
9145-I716055(built): AHSP in 9145(biobricks 2.0 format)
Cassette Construction Using 1128 RBS Library:
9145-I716056(built): -kan-RBS library-alpha HbA-
9145-I716057(built): -CmR-RBS library-beta HbA-
9145-I716060(built): beta-alpha cassette (with RBS libraries)
9145-I716065(built): 2X mutant -Kan-rbs-alpha Hba (L29F and V96W)
9145-I716066(built): 2X mutant -Cmr-beta Hba (Asn108Lys and Lys82Asp)
9145-I716067(built): 2X mutants -rbs library-beta-rbs library-alpha- (with RBS libraries)
9145-I716061(built): pTRC-rbs-beta-rbs-alpha
pTrc-I716069(built): 2X mutant -pTRC-rbs-2x beta-rbs-2x alpha L29F/V96W and Asn108Lys/Lys82Asp
HbA Subunit Mutant Variants:
9145-I716058(built): 1X mutant HbA alpha (L29F) AKA: I716053m29
9145-I716059(built): 1X mutant HbA beta (Asn108Lys "Presbyterian") AKA: I716054m108
9145-I716062(built): 2X mutant HbA alpha (L29F/V96W) AKA: I716053m29m96
9145-I716063(built): 2X mutant HbA beta (Asn108Lys/Lys82Asp "Providence/Presbyterian") AKA: I716054m108m82
9145-I716072(built): 1X mutant beta Hba (Lys82Asp "Providence")
9145-I716073(built): unmutated Di-alpha fusion, biobricked
9145-I716068(built): 2X mutant Di-Alpha fusion, biobricked
No Scar Rbs-Part RBS library
1103-I716064: -pCon-(no scar site)-rbs-beta-rbs-alpha
9145-I716070(built): alpha HbA-TT
9145-I716077(built): Unmutated Di-Alpha fusion - TT
9145-I716078(built): 2X mutant alpha - TT
9145-I716079(built): 2X mutant Di-alpha fusion -TT
9145-I716080(built): -rbs-(no scar)-beta
9145-I716081(built): -rbs-(no scar)-1X mutant beta (providence Lys82Asp)
9145--I716082(built): -rbs-(no scar)-2X mutant beta (providence/Presbyterian Lys82Asp/Asn108Lys)
9145-I716071(built): -rbs lib-(NO SCAR)-beta-rbs lib-(no scar)-alpha-TT
9145-I716074(built): -rbs lib-(NO SCAR)-beta 1X mutant, providence (Lys82Asp) - rbs lib-(no scar) - unmutated di-alpha-TT- (Best Yield)
9145-I716075(built): -rbs lib-(NO SCAR)-beta 2X mutant, providence (Lys82Asp) and Presbyterian (Asn108Lys)-rbs lib-(no scar) - unmutated di alpha - TT- (Best all around)
9145-I716076(built): -rbs lib-(NO SCAR)-1X mutant beta, presbyterian (Asn108Lys)-rbs lib-(no scar)-2X mutant alpha (L29F and V96W) - TT (Second best all around)
9145-I716083(built): pT7105-rbs lib-(NO SCAR)-beta-rbs lib-(no scar)-alpha-TT
9145-I716084(built): pT7105-rbs lib-(NO SCAR)-beta 1X mutant, providence (Lys82Asp) - rbs lib-(no scar) - unmutated di-alpha-TT- (Best Yield)
9145-I716085(built): pT7105-rbs lib-(NO SCAR)-beta 2X mutant, providence (Lys82Asp) and Presbyterian (Asn108Lys)-rbs lib-(no scar) - unmutated di alpha - TT- (Best all around)
9145-I716086(built): pT7105-rbs lib-(NO SCAR)-1X mutant beta, presbyterian (Asn108Lys)-rbs lib-(no scar)-2X mutant alpha (L29F and V96W) - TT (Second best all around)
petDUET stuffs beta insertion
9145-I716087(built): (unmodified beta)insertion
9145-I716088(built): (Beta providence) insertion
9145-I716089(built): (Beta providence / Presbyterian) insertion
9145-I716090(built): (Beta presbyterian) insertion
petDUET stuffs alpha and beta insertion
9145-I716091(built): unmodified beta + unmodified alpha: "Unmodified" in petDUET
9145-I716092: Beta providence + unmodified dialpha fusion: "Best Yield" in petDUET
9145-I716093(built): Beta providence / Presbyterian + unmodified dialpha fusion : "Best All Around" in petDUET
9145-I716094(built): Beta presbyterian + 2X mutant alpha L29F/V96W: "Second Best All Around" in petDUET
(Basically Biobricked)Hemoglobin cassette + hemA/hemB:
9145-I716095 (built): "Unmodified" (I716091) + hemA/hemB cassette
9145-I716096 (built): "Best Yield" (I716092) + hemA/hemB cassette
9145-I716097 (built): "Best All Around" (I716093) + hemA/hemB cassette
9145-I716098: "Second Best All Around" (I716094) + hemA/hemB cassette
(Basically Biobricked)Hemoglobin cassette + Cytochrome B5/B5 reductase cassette + hemA/hemB cassette:
9145-I716099(built): "Unmodified" (I716091) + cytochrome b5/b5reductase cassette + hemA/hemB cassette
9145-I716100: "Best Yield" (I716092) + cytochrome b5/b5 reductase cassette + hemA/hemB cassette
9145-I716351(built): "Best All Around" (I716093) + cytochrome b5/b5 reductase cassette + hemA/hemB cassette
9145-I716352: "Second Best All Around" (I716094) + cytochrome b5/b5reductase cassette + hemA/hemB cassette
(Basically Biobricked)Hemoglobin cassette + cytochrome b5/b5 reductase + hemA/hemB + sodC/katG cassette:
9145-I716358: "Unmodified" (I716091) + cytochrome b5/b5reductase cassette + hemA/hemB cassette + sodC/KatG
9145-I716359: "Best Yield" (I716092) + cytochrome b5/b5 reductase cassette + hemA/hemB cassette + sodC/katG
9145-I716360: "Best All Around" (I716093) + cytochrome b5/b5 reductase cassette + hemA/hemB cassette + sodC/KatG
9145-I716361: "Second Best All Around" (I716094) + cytochrome b5/b5reductase cassette + hemA/hemB cassette + sodC/KatG
Detox constructs
9145-I716353: AdhE in petDUET
Biobricked petDUET Hemoglobin Cassettes (XhoI/EcoRI/BglII sites removed)
9145-I716354: "Unmodified" (I716091)
9145-I716355: "Best Yield" (I716092)
9145-I716356: "Best All Around" (I716093)
9145-I716357: "Second Best All Around" (I716094)
Different T7's with Hem Genes
9145-I716366: T7 Clone A with Hem genes A + B
9145-I716367: T7 Clone C with Hem genes A + B
Biobricked petDUET Hemoglobin Cassettes + sodC/katG (Line discontinued)
9145-I716362: Unmodified
9145-I716363: "Best Yield" (Mutant Line Discontinuted)
9145-I716364: Best All Around
9145-I716365: "Second Best All Around" (Mutant Line Discontinuted)
Biobricked petDUET Hemoglobin + SodC/KatG + Cytochrome b5/b5 reductase (Line discontinued)
9145-I716368: Unmodified cassette with antioxidants + cytochromes
9145-I716369: Best all around cassette with antioxidants + cytochromes
Biobricked petDUET Hemoglobin + sodC/katG + Cytochromes + AHSP(Line discontinued)
9145-I716370: Unmodified cassette
9145-I716371: Best all around cassette
Biobricked petDUET Hemoglobin + sodC/katG + Cytochromes + AHSP + metAP(Line discontinued)
9145-I716372: Unmodified cassette
9145-I716373: Best all around cassette
Biobricked petDUET Hemoglobin Cassettes + heme
9145-I716374: Unmodified cassette
9145-I716375: Best all around cassette
Biobricked petDUET Hemoglobin Cassettes + heme + Cytochromes
9145-I716376: Unmodified cassette
9145-I716377: Best all around cassette
Biobricked petDUET Hemoglobin Cassettes + heme + AHSP
9145-I716378: Unmodified cassette
9145-I716379: Best all around cassette
Biobricked petDUET Hemoglobin Cassettes + heme + sodC/katG
9145-I716380: Unmodified cassette
9145-I716381: Best all around cassette
Biobricked petDUET Hemoglobin + heme + cytochromes + sodC/katG
9145-I716383: Unmodified
9145-I716384: Best all around
Biobricked petDUET Hemoglobin + heme + cytochromes + sodC/katG + AHSP
9145-I716385: Unmodified
9145-I716386: Best all around
Biobricked petDUET Hemoglobin + heme + cytochromes + sodC/katG + AHSP + metAP
9145-I716386: Unmodified Final Construct
9145-I716387: Best all around Final Construct
Biobricked petDUET Hemoglobin + heme + sodC/katG + AHSP + metAP
9145-I716388: Unmodified
9145-I716389: Best all around
HemeABCD in pBACb
9145-I716382: pBACb with hemABCD
HemeABCD-TT in 9145
9145-I716390: HemABCD-TT in 9145
Unused Numbers
