Template:BerkiGEM2007 SamanthaConstructionFiles

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Construction of pBca9125-I716201, I716202, I716203, I716204 (rbs+ATG variants)
Construction of pBca9125-I716205, I716206 (lox+Term variants)
Construction of pBca9125-I716207, I716208 (lox+Term+lox variants)
Construction of pBca9125-I716209 (ceaB biobrick)
Construction of pBca9125-I716210 (BamHI biobrick)
Construction of pBca9125-I716211 (Barnase biobrick)
Construction of pBca9125-I716212, I716213 (Cre biobricks with various start codons)
Construction of pBca9125-I716214, I716215 (rbs+ATG+Lox+Term+lox cassettes)
Construction of pBca9125-I716216, I716217 (cassettes+ceaB)
Construction of pBca9125-I716218, I716219 (cassettes+BamHI)
Construction of pBca9125-I716220, I716221 (cassettes+Barnase)
Construction of pBca9125-I716222, I716223 (cassettes+BglII)
Construction of pBca9125-I716224, I716225, I716226 (rbs library+Cre variants)
Construction of all [rbs][Cre][rbs][ATG][lox][term][lox][toxin] variants (I716227-I716250)
Construction of all [pBad][rbs][Cre][rbs][ATG][lox][term][lox][toxin] variants (I716401-I716415, plus I716416-I716421)
Construction of pBca9125-I716422, I716423 (cassettes+RFP)
Construction of pBca9125-I716424 through I716429 ([rbs][Cre][rbs][ATG][lox][term][lox][RFP] variants)
Construction of all [pBad][rbs][Cre][rbs][ATG][lox][term][lox][RFP] variants (I716430-I716435)
Construction of pBca1101-I716436 and pBca9125-I716437 (rbs+Cre variants)
Construction of pBca1101-I716438 and pBca9125-I716439 (rbs+Cre+TT variants)
Construction of pBca1101-I716440 (Cre+TTcassette+barnase)
Construction of pBca1101-I716441 (pbad.1106Arbs)
Construction of pBca1101-I716442 (pbad.1106Arbs.Cre.TTcassette.barnase)
Construction of pBca1101-I716443 and pBca9125-I716444 (rbs+Cre+TT+lox variants)
Construction of pBca1101-I716445 and pBca9125-I716446 (lox+rbs+Cre+TT+lox variants)
Construction of pBca1101-I716447 and pBca9125-I716448 (pbad+1090rbs+lox+rbs+Cre+TT+lox variants)
Construction of pBca9145-I716449 (pbad.1090rbs.ATG)
Construction of pBca9145-I716450 (pbad.1106Arbs.ATG)
Construction of pBca9125-I716451 through I716454 ([pbad][1090][ATG][toxin] variants)
Construction of pBca9125-I716455 through I716458 ([pbad][1106A][toxin] variants)

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