Imperial/Wet Lab/Lab Notebook/2007-10-19
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==AHL titration curve for infector detector construct== | ==AHL titration curve for infector detector construct== | ||
*The '''aim'' of this experiment was to test the pTet-luxR-pLux-GFPmut3b construct with a range of AHL concentrations. A titration curve could then be created for the construct in terms of AHL concentrations | *The '''aim'' of this experiment was to test the pTet-luxR-pLux-GFPmut3b construct with a range of AHL concentrations. A titration curve could then be created for the construct in terms of AHL concentrations | ||
Revision as of 18:57, 26 October 2007
AHL titration curve for infector detector construct
- The 'aim of this experiment was to test the pTet-luxR-pLux-GFPmut3b construct with a range of AHL concentrations. A titration curve could then be created for the construct in terms of AHL concentrations
- The materials and methods can be found in the Protocols
- The concentrations of AHL for which the construct was tested are: 0nM, 5nM, 10nM, 15nM, 20nM, 50nM, 100nM, 1000nM
- There were two negative controls for the experiment:
- Empty vector with [AHL]=1000nM
- pTet-luxR-pLux-GFPmut3b construct with [AHL] = 0nM
- The Results showed that the total fluorescent output produced by the construct increases with increasing [AHL]
