1 August 2007

Preparation of Stock Solutions

• 1x 1 litre of 10x TBE
• 20x 100 ml of LB media in conical flasks

Preparation of Competent Cells

1. Poured LB agar plates
   • Nalixidic acid (15 μg/ml)
   • Tetracyclin (20 μg/ml)
2. Streaked E.coli cells on agar plates
   • DH5Alpha
   • XL1Blue
3. Incubated plates at 37°C overnight

Preparation of Equipment

• 1x 37°C shaking incubator
• 1x 4°C fridge
• 1x -20°C freezer
• 2x Weighing scales
• 3x Magnetic stirrer
• 3x Microcentrifuge
• 3x Vortex machines
• 2x UV Spectrometer
• 5x Gilson pipettes (P1000, P200, P20, P10, P2)
• 20 boxes x Tips (yellow and blue)
• 5 boxes x Small tips
• Eppendorf tubes
• Centrifuge tubes
• 50x 5 ml pipettes
• 50x 10 ml pipettes
• 50x 25 ml pipettes
• 5x Pipette fillers
• 37°C water bath
• 15°C water bath
• Driblock
• 10 packets x Innoculation loops
• 3x Bunsen burners
• 3 boxes Matches
• 3x Agarose gel equipments
• Microwave oven
• Transilluminator
• Camera
• 3x Scapel (for cutting agarose gel)
• 1 litre bottles
• Beakers
• Conical flasks
• Volumetric flasks
• Measuring cylinders
• Gloves
• Goggles
• Markers
• Sticker labels
• Paper towels