Imperial/Wet Lab/Lab Notebook/2007-10-19


AHL titration curve for infector detector construct

  • The 'aim of this experiment was to test the pTet-luxR-pLux-GFPmut3b construct with a range of AHL concentrations. A titration curve could then be created for the construct in terms of AHL concentrations
  • The materials and methods can be found in the Protocols
  • The concentrations of AHL for which the construct was tested are: 0nM, 5nM, 10nM, 15nM, 20nM, 50nM, 100nM, 1000nM
  • There were two negative controls for the experiment:
    • Empty vector with [AHL]=1000nM
    • pTet-luxR-pLux-GFPmut3b construct with [AHL] = 0nM
  • The Results showed that the total fluorescent output produced by the construct increases with increasing [AHL]