Imperial/Wet Lab/Lab Notebook/2007-10-16


pT7-GFPmut3b chracterisation

  • The aim of today's test was to chracterise the pT7-GFPmut3b with respect to temperature
  • For materials and methods see the Protocol
  • The construct was tested for three temperatures: 4°C, 25°C and 37°C
  • The reactions were sampled every hour
  • The Results showed that pT7-GFPmut3b expressed at all three temperatures and the fluorescence is the highest for greater temperatures