2 October 2007

Test purified luxR

• Aim: The aim of this experiment was to test if the purified luxR works and if the construct 2 works
• To test the purified luxR we used these Protocol
• The results of this experiment can be found Results
• The purified LuxR appeared to be functional in vitro, however there were stability issues that reduce the synthesis of GFPmut3b when pLux-GFPmut3b is used with purified LuxR.
• For the characterisation of the infector detector system the pTet-LuxR-pLux-GFPmut3b (construct 1) will be used.

Test pT7 in-vivo

• Aim: The aim of the test was to verify that pT7-GFP expresses in-vivo
• The test was carried out using these protocols Protocol
• The results can be found here Results