Imperial/Wet Lab/Lab Notebook/2007-08-23

From 2007.igem.org

23 August 2007

Preparation of Reaction Buffer for S30 Cell Extract

  1. Calculated and weighed out required amounts of reagents
  2. Left mixture on the stirrer in the cold room overnight
    • Phosphoenolpyruvate to be added tomorrow

Protocols can be found at [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Notebook/General_Protocols#S30.2FS12_Cell_Extract#S30/S12_Cell_Extract| S30/S12 Cell Extract] in the general protocols page

Vesicles Formation with GFP

Preparations

The desiccation and suspension stages of the vesicle preparation protocol were followed.

  • One 100ml beaker was prepared with 0.5mg of powdered POPC in 50ml of dodecane.
  • The suspension was sonicated for 30mins and left overnight in a 27°C incubator.