Imperial/Wet Lab/Lab Notebook/2007-08-10

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Protocols can be found at [[IGEM:IMPERIAL/2007/Notebook/General_Protocols#Miniprep|Miniprep]] in the general protocols page
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Protocols can be found at [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Notebook/General_Protocols#Miniprep Miniprep] in the general protocols page
==Agarose Gel Electrophoresis of Biobricks==
==Agarose Gel Electrophoresis of Biobricks==
#Checked 2x 14 Biobricks on 1% agarose gel
#Checked 2x 14 Biobricks on 1% agarose gel
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Protocols can be found at [[IGEM:IMPERIAL/2007/Notebook/General_Protocols#Electrophoresis|Electrophoresis]] in the general protocols page
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Protocols can be found at [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Notebook/General_Protocols#Electrophoresis Electrophoresis] in the general protocols page
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Latest revision as of 19:36, 26 October 2007

10 August 2007

Preparation of Stock Solutions

  • 1x 500 ml of 2M Tris-acetate pH 8.2
  • 1x 500 ml of Buffer A for preparation of S30/S12 cell extract
  • 1x 500 ml of Buffer B for preparation of S30/S12 cell extract
  • 1x 50 ml of 2mM amino acids

Miniprep of Biobricks

  1. Minipreped 2x 14 Biobricks

Protocols can be found at [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Notebook/General_Protocols#Miniprep Miniprep] in the general protocols page

Agarose Gel Electrophoresis of Biobricks

  1. Checked 2x 14 Biobricks on 1% agarose gel

Protocols can be found at [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Notebook/General_Protocols#Electrophoresis Electrophoresis] in the general protocols page

IC07 Gel 1.JPG IC07 Gel 3.JPG IC07 Gel 2.JPG