Imperial/Wet Lab/Lab Notebook/2007-09-02
From 2007.igem.org
< Imperial | Wet Lab | Lab Notebook
2 September 2007
Construction of pT7-GFP
- 5 colonies grew in the ligation plate
- All 5 colonies were picked and each was grown in 5 ml of LB + Kan
- The 5 cultures were incubated at 37°C overnight
Cloning of Biobricks (for Maxiprep)
- Placed 200 ml of LB in a 500 ml conical flask
- Innoculated 2 ml of culture into LB media in flask
- Incubated overnight at 37 °C
- 2. BBa_I13422 [ptet-GFP]
- 18. BBa_T9002 [plux-GFP]
