Imperial/Wet Lab/Lab Notebook/2007-10-02
From 2007.igem.org
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= 2 October 2007 = | = 2 October 2007 = | ||
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| + | ==Evaporation experient== | ||
| + | *We carried on experiments from yesterday to find a method to reduce evaporation of samples | ||
| + | *Results??? | ||
| + | |||
| + | ==Test purified luxR== | ||
| + | *'''Aim''': The aim of this experiment was to test if the purified luxR works and if the construct 2 works | ||
| + | *To test the purified luxR we used these [[IGEM:IMPERIAL/2007/Wet Lab/Protocols/Prot1.7|protocols]] | ||
| + | *The results of this experiment can be found [[IGEM:IMPERIAL/2007/new pages/Construct 2|here]] | ||
| + | *The purified LuxR appeared to be functional in vitro, however there were stability issues that reduce the synthesis of GFPmut3b when pLux-GFPmut3b is used with purified LuxR. | ||
| + | *For the characterisation of the infector detector system the pTet-LuxR-pLux-GFPmut3b (construct 1) will be used. | ||
| + | |||
| + | ==Test pT7 in-vivo== | ||
| + | *'''Aim''': The aim of the test was to verify that pT7-GFP expresses in-vivo | ||
| + | *The test was carried out using these protocols?? | ||
| + | *The results can be found here?? | ||
Revision as of 19:43, 24 October 2007
Contents |
2 October 2007
Evaporation experient
- We carried on experiments from yesterday to find a method to reduce evaporation of samples
- Results???
Test purified luxR
- Aim: The aim of this experiment was to test if the purified luxR works and if the construct 2 works
- To test the purified luxR we used these protocols
- The results of this experiment can be found here
- The purified LuxR appeared to be functional in vitro, however there were stability issues that reduce the synthesis of GFPmut3b when pLux-GFPmut3b is used with purified LuxR.
- For the characterisation of the infector detector system the pTet-LuxR-pLux-GFPmut3b (construct 1) will be used.
Test pT7 in-vivo
- Aim: The aim of the test was to verify that pT7-GFP expresses in-vivo
- The test was carried out using these protocols??
- The results can be found here??
