Imperial/Wet Lab/Lab Notebook/2007-10-02

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__NOTOC__
= 2 October 2007 =
= 2 October 2007 =
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==Evaporation experient==
 
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*We carried on experiments from yesterday to find a method to reduce evaporation of samples
 
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*Results???
 
==Test purified luxR==
==Test purified luxR==
*'''Aim''': The aim of this experiment was to test if the purified luxR works and if the construct 2 works
*'''Aim''': The aim of this experiment was to test if the purified luxR works and if the construct 2 works
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*To test the purified luxR we used these [[IGEM:IMPERIAL/2007/Wet Lab/Protocols/Prot1.7|protocols]]
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*To test the purified luxR we used these [[Imperial/Wet_Lab/Protocols/Prot1.7|Protocol]]
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*The results of this experiment can be found [[IGEM:IMPERIAL/2007/new pages/Construct 2|here]]
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*The results of this experiment can be found [[Imperial/Wet_Lab/Results/ID1.7|Results]]
*The purified LuxR appeared to be functional in vitro, however there were stability issues that reduce the synthesis of GFPmut3b when pLux-GFPmut3b is used with purified LuxR.  
*The purified LuxR appeared to be functional in vitro, however there were stability issues that reduce the synthesis of GFPmut3b when pLux-GFPmut3b is used with purified LuxR.  
*For the characterisation of the infector detector system the pTet-LuxR-pLux-GFPmut3b (construct 1) will be used.
*For the characterisation of the infector detector system the pTet-LuxR-pLux-GFPmut3b (construct 1) will be used.
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==Test pT7 in-vivo==
==Test pT7 in-vivo==
*'''Aim''': The aim of the test was to verify that pT7-GFP expresses in-vivo
*'''Aim''': The aim of the test was to verify that pT7-GFP expresses in-vivo
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*The test was carried out using these protocols??
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*The test was carried out using these protocols [[Imperial/Wet_Lab/Protocols/Prot1.9|Protocol]]
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*The results can be found here??
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*The results can be found here [[Imperial/Wet_Lab/Results/Res1.9|Results]]

Latest revision as of 20:51, 26 October 2007

2 October 2007

Test purified luxR

  • Aim: The aim of this experiment was to test if the purified luxR works and if the construct 2 works
  • To test the purified luxR we used these Protocol
  • The results of this experiment can be found Results
  • The purified LuxR appeared to be functional in vitro, however there were stability issues that reduce the synthesis of GFPmut3b when pLux-GFPmut3b is used with purified LuxR.
  • For the characterisation of the infector detector system the pTet-LuxR-pLux-GFPmut3b (construct 1) will be used.

Test pT7 in-vivo

  • Aim: The aim of the test was to verify that pT7-GFP expresses in-vivo
  • The test was carried out using these protocols Protocol
  • The results can be found here Results