Imperial/Wet Lab/Lab Notebook/2007-10-10
From 2007.igem.org
< Imperial | Wet Lab | Lab Notebook(Difference between revisions)
m (→Media test) |
m (→pT7 test in-vitro) |
||
| (One intermediate revision not shown) | |||
| Line 8: | Line 8: | ||
*The '''aim''' of this test was to see if the fluorescence of GFP mut3b gets affected by the media it is in. | *The '''aim''' of this test was to see if the fluorescence of GFP mut3b gets affected by the media it is in. | ||
*We tested the reporter's expression in nuclease free water and LB media and then compared it with the reults got from GFP mut3b calibration curve | *We tested the reporter's expression in nuclease free water and LB media and then compared it with the reults got from GFP mut3b calibration curve | ||
| - | *The results can be found [[Media | + | *The results can be found [[Media:IC 2007 Mediatest.xls|here]] |
*It was found a linear relationship between the fluorescence of GFP mut3b in the three media. | *It was found a linear relationship between the fluorescence of GFP mut3b in the three media. | ||
*This meant that LB media could be used to get calibration curve for dsRED express instead of cell extract. But as dsRED express couldn't be cloned, we didn't make a calibration curve for dsRED express | *This meant that LB media could be used to get calibration curve for dsRED express instead of cell extract. But as dsRED express couldn't be cloned, we didn't make a calibration curve for dsRED express | ||
| Line 14: | Line 14: | ||
==pT7 test in-vitro== | ==pT7 test in-vitro== | ||
*The '''aim''' of the test was to see if pT7-GFP mut3b and pT7-dsRED express work in-vitro or not | *The '''aim''' of the test was to see if pT7-GFP mut3b and pT7-dsRED express work in-vitro or not | ||
| - | *The protocols can be found [[ | + | *The protocols can be found [[Imperial/Wet_Lab/Protocols/Prot1.9|Protocol]] |
*The experiment didn't give good results as: | *The experiment didn't give good results as: | ||
**The incubator had been turned off, thus the samples were not at the right temperature. | **The incubator had been turned off, thus the samples were not at the right temperature. | ||
**The right filter for dsRED express was not used to begin with | **The right filter for dsRED express was not used to begin with | ||
*After using the right filter, it was realised that pT7-dsRED express construct doesn't express | *After using the right filter, it was realised that pT7-dsRED express construct doesn't express | ||
| - | *The results can be found [[ | + | *The results can be found [[Imperial/Wet_Lab/Results/Res1.9|Results]] |
<html></div></html> | <html></div></html> | ||
{{Template:IC07labnotebook}} | {{Template:IC07labnotebook}} | ||
Latest revision as of 18:54, 26 October 2007
10 October 2007
Media test
- The aim of this test was to see if the fluorescence of GFP mut3b gets affected by the media it is in.
- We tested the reporter's expression in nuclease free water and LB media and then compared it with the reults got from GFP mut3b calibration curve
- The results can be found here
- It was found a linear relationship between the fluorescence of GFP mut3b in the three media.
- This meant that LB media could be used to get calibration curve for dsRED express instead of cell extract. But as dsRED express couldn't be cloned, we didn't make a calibration curve for dsRED express
pT7 test in-vitro
- The aim of the test was to see if pT7-GFP mut3b and pT7-dsRED express work in-vitro or not
- The protocols can be found Protocol
- The experiment didn't give good results as:
- The incubator had been turned off, thus the samples were not at the right temperature.
- The right filter for dsRED express was not used to begin with
- After using the right filter, it was realised that pT7-dsRED express construct doesn't express
- The results can be found Results
