Imperial/Wet Lab/Lab Notebook/2007-08-01
From 2007.igem.org
(Difference between revisions)
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= 1 August 2007 = | = 1 August 2007 = | ||
| + | ===Preparation of Stock Solutions=== | ||
| + | *1x 1 litre of 10x TBE | ||
| + | *20x 100 ml of LB media in conical flasks | ||
| + | |||
| + | ===Preparation of Competent Cells=== | ||
| + | #Poured LB agar plates | ||
| + | #*Nalixidic acid (15 μg/ml) | ||
| + | #*Tetracyclin (20 μg/ml) | ||
| + | #Streaked E.coli cells on agar plates | ||
| + | #*DH5Alpha | ||
| + | #*XL1Blue | ||
| + | #Incubated plates at 37°C overnight | ||
| + | |||
| + | ===Preparation of Equipment=== | ||
| + | *1x 37°C shaking incubator | ||
| + | *1x 4°C fridge | ||
| + | *1x -20°C freezer | ||
| + | *2x Weighing scales | ||
| + | *3x Magnetic stirrer | ||
| + | *3x Microcentrifuge | ||
| + | *3x Vortex machines | ||
| + | *2x UV Spectrometer | ||
| + | *5x Gilson pipettes (P1000, P200, P20, P10, P2) | ||
| + | *20 boxes x Tips (yellow and blue) | ||
| + | *5 boxes x Small tips | ||
| + | *Eppendorf tubes | ||
| + | *Centrifuge tubes | ||
| + | *50x 5 ml pipettes | ||
| + | *50x 10 ml pipettes | ||
| + | *50x 25 ml pipettes | ||
| + | *5x Pipette fillers | ||
| + | *37°C water bath | ||
| + | *15°C water bath | ||
| + | *Driblock | ||
| + | *10 packets x Innoculation loops | ||
| + | *3x Bunsen burners | ||
| + | *3 boxes Matches | ||
| + | *3x Agarose gel equipments | ||
| + | *Microwave oven | ||
| + | *Transilluminator | ||
| + | *Camera | ||
| + | *3x Scapel (for cutting agarose gel) | ||
| + | *1 litre bottles | ||
| + | *Beakers | ||
| + | *Conical flasks | ||
| + | *Volumetric flasks | ||
| + | *Measuring cylinders | ||
| + | *Gloves | ||
| + | *Goggles | ||
| + | *Markers | ||
| + | *Sticker labels | ||
| + | *Paper towels | ||
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Revision as of 10:48, 9 October 2007
Contents |
1 August 2007
Preparation of Stock Solutions
- 1x 1 litre of 10x TBE
- 20x 100 ml of LB media in conical flasks
Preparation of Competent Cells
- Poured LB agar plates
- Nalixidic acid (15 μg/ml)
- Tetracyclin (20 μg/ml)
- Streaked E.coli cells on agar plates
- DH5Alpha
- XL1Blue
- Incubated plates at 37°C overnight
Preparation of Equipment
- 1x 37°C shaking incubator
- 1x 4°C fridge
- 1x -20°C freezer
- 2x Weighing scales
- 3x Magnetic stirrer
- 3x Microcentrifuge
- 3x Vortex machines
- 2x UV Spectrometer
- 5x Gilson pipettes (P1000, P200, P20, P10, P2)
- 20 boxes x Tips (yellow and blue)
- 5 boxes x Small tips
- Eppendorf tubes
- Centrifuge tubes
- 50x 5 ml pipettes
- 50x 10 ml pipettes
- 50x 25 ml pipettes
- 5x Pipette fillers
- 37°C water bath
- 15°C water bath
- Driblock
- 10 packets x Innoculation loops
- 3x Bunsen burners
- 3 boxes Matches
- 3x Agarose gel equipments
- Microwave oven
- Transilluminator
- Camera
- 3x Scapel (for cutting agarose gel)
- 1 litre bottles
- Beakers
- Conical flasks
- Volumetric flasks
- Measuring cylinders
- Gloves
- Goggles
- Markers
- Sticker labels
- Paper towels
