Imperial/Wet Lab/Lab Notebook/2007-08-02
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< Imperial | Wet Lab | Lab Notebook(Difference between revisions)
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| + | ==Preparation of Stock Solutions== | ||
| + | * 1x 1 litre of 1M Tris-Cl | ||
| + | * 1x 500 ml of 50x Tris-acetate | ||
| + | ==Preparation of Competent Cells== | ||
| + | #Picked colonies from agar plates | ||
| + | #Grew colonies in LB media | ||
| + | #*DH5Alpha | ||
| + | #*XL1Blue | ||
| + | #Incubated cultures at 37°C overnight | ||
Latest revision as of 13:34, 10 October 2007
2 August 2007
Preparation of Stock Solutions
- 1x 1 litre of 1M Tris-Cl
- 1x 500 ml of 50x Tris-acetate
Preparation of Competent Cells
- Picked colonies from agar plates
- Grew colonies in LB media
- DH5Alpha
- XL1Blue
- Incubated cultures at 37°C overnight
