Imperial/Wet Lab/Lab Notebook/2007-08-08
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| + | ==Transformation of Biobricks== | ||
| + | #15 biobricks were transformed into XL1-Blue | ||
| + | #Cells were grown for 1 hour and plated | ||
| + | #Plates were at 37°C overnight | ||
| + | |||
| + | <font size=-2> | ||
| + | *1. BBa_E0040 [GFP] | ||
| + | *2. BBa_I13352 [ptet-GFP] | ||
| + | *3. BBa_J5528 [pBad-GFP] | ||
| + | *4. BBa_J24677 [ptet-LuxR-plux-GFP] | ||
| + | *5. BBa_R0040 [ptet] | ||
| + | *6. BBa_I0500 [pBad] | ||
| + | *7. BBa_F2620 [ptet-LuxR-plux] | ||
| + | *8. BBa_F1610 [luxI] | ||
| + | *9. BBa_R0010 [lacI] | ||
| + | *10.BBa_E7010 [RFP] | ||
| + | *11.BBa_E7104 [T7 GFP] | ||
| + | *12.BBa_S03550 [araC] | ||
| + | *13.BBa_B0015 [Terminator] | ||
| + | *14.BBa_R0051 [pCI] | ||
| + | *15.BBa_S07037 [pCI-GFP] | ||
| + | </font> | ||
| + | |||
| + | Protocols can be found at [[IGEM:IMPERIAL/2007/Notebook/General_Protocols#Electroporation|Electroporation]] in the general protocols page | ||
| + | |||
| + | *Plate 3 had no colony (possibly due to large plasmid size - difficult to transform) | ||
| + | |||
| + | ==Preparation of Competent Cells== | ||
| + | #BL21(DE3)-Gold was made electrocompetent | ||
| + | Protocols can be found at [[IGEM:IMPERIAL/2007/Notebook/General_Protocols#Preparation_of_Competent_Cells|Preparation of Competent Cells]] in the general protocols page | ||
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{{Template:IC07labnotebook}} | {{Template:IC07labnotebook}} | ||
Revision as of 13:36, 10 October 2007
8 August 2007
Transformation of Biobricks
- 15 biobricks were transformed into XL1-Blue
- Cells were grown for 1 hour and plated
- Plates were at 37°C overnight
- 1. BBa_E0040 [GFP]
- 2. BBa_I13352 [ptet-GFP]
- 3. BBa_J5528 [pBad-GFP]
- 4. BBa_J24677 [ptet-LuxR-plux-GFP]
- 5. BBa_R0040 [ptet]
- 6. BBa_I0500 [pBad]
- 7. BBa_F2620 [ptet-LuxR-plux]
- 8. BBa_F1610 [luxI]
- 9. BBa_R0010 [lacI]
- 10.BBa_E7010 [RFP]
- 11.BBa_E7104 [T7 GFP]
- 12.BBa_S03550 [araC]
- 13.BBa_B0015 [Terminator]
- 14.BBa_R0051 [pCI]
- 15.BBa_S07037 [pCI-GFP]
Protocols can be found at Electroporation in the general protocols page
- Plate 3 had no colony (possibly due to large plasmid size - difficult to transform)
Preparation of Competent Cells
- BL21(DE3)-Gold was made electrocompetent
Protocols can be found at Preparation of Competent Cells in the general protocols page
