Imperial/Wet Lab/Lab Notebook/2007-10-19
From 2007.igem.org
< Imperial | Wet Lab | Lab Notebook(Difference between revisions)
| (3 intermediate revisions not shown) | |||
| Line 1: | Line 1: | ||
| + | {{Template:IC07navmenu}} | ||
| + | <html><div id="maincol"></html> | ||
| + | __NOTOC__ | ||
| + | |||
| + | |||
==AHL titration curve for infector detector construct== | ==AHL titration curve for infector detector construct== | ||
*The '''aim'' of this experiment was to test the pTet-luxR-pLux-GFPmut3b construct with a range of AHL concentrations. A titration curve could then be created for the construct in terms of AHL concentrations | *The '''aim'' of this experiment was to test the pTet-luxR-pLux-GFPmut3b construct with a range of AHL concentrations. A titration curve could then be created for the construct in terms of AHL concentrations | ||
| Line 7: | Line 12: | ||
**pTet-luxR-pLux-GFPmut3b construct with [AHL] = 0nM | **pTet-luxR-pLux-GFPmut3b construct with [AHL] = 0nM | ||
*The [[Imperial/Wet Lab/Results/ID3.1|Results]] showed that the total fluorescent output produced by the construct increases with increasing [AHL] | *The [[Imperial/Wet Lab/Results/ID3.1|Results]] showed that the total fluorescent output produced by the construct increases with increasing [AHL] | ||
| + | |||
| + | |||
| + | |||
| + | <html></div></html> | ||
| + | {{Template:IC07labnotebook}} | ||
Latest revision as of 18:59, 26 October 2007
AHL titration curve for infector detector construct
- The 'aim of this experiment was to test the pTet-luxR-pLux-GFPmut3b construct with a range of AHL concentrations. A titration curve could then be created for the construct in terms of AHL concentrations
- The materials and methods can be found in the Protocols
- The concentrations of AHL for which the construct was tested are: 0nM, 5nM, 10nM, 15nM, 20nM, 50nM, 100nM, 1000nM
- There were two negative controls for the experiment:
- Empty vector with [AHL]=1000nM
- pTet-luxR-pLux-GFPmut3b construct with [AHL] = 0nM
- The Results showed that the total fluorescent output produced by the construct increases with increasing [AHL]
