Imperial/Wet Lab/Lab Notebook/2007-09-07
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| + | == LuxR Purification == | ||
| + | [[Image:icgems_LuxR-sds1.png|left|thumb|340px|SDS-PAGE done. Columns: Gel Marker, soluble (diluted), soluble, insoluble (dilted), insoluble]] | ||
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| + | We did a preliminary test on two protocols that were used to purify LuxR. One involve the extraction of LuxR in soluble form, while the other was the extraction of LuxR in the form of inclusion bodies. | ||
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| + | The results suggest that soluble form protocol appears to yield increased amounts of LuxR protein (29kDa). This corresponds near to the marker set at 31kDa (the 5th line on the marker). | ||
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| + | == Vesicles == | ||
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| + | '''Results''' <br> | ||
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| + | Sample 8, produced on [[Imperial/Wet Lab/Lab Notebook/2007-09-04|04.09.2007]] was looked at again under the fluorescence microscope, and vesicles were still present at a density similar to that of the first observation. | ||
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Latest revision as of 15:24, 26 October 2007
7 September 2007
LuxR Purification
We did a preliminary test on two protocols that were used to purify LuxR. One involve the extraction of LuxR in soluble form, while the other was the extraction of LuxR in the form of inclusion bodies.
The results suggest that soluble form protocol appears to yield increased amounts of LuxR protein (29kDa). This corresponds near to the marker set at 31kDa (the 5th line on the marker).
Vesicles
Results
Sample 8, produced on 04.09.2007 was looked at again under the fluorescence microscope, and vesicles were still present at a density similar to that of the first observation.
