Imperial/Wet Lab/Lab Notebook/2007-09-10
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'''Results''' <br> | '''Results''' <br> | ||
| - | Sample 8, produced on [[ | + | Sample 8, produced on [[Imperial/Wet Lab/Lab Notebook/2007-09-04|04.09.2007]] was looked at again under the fluorescence microscope, but this time only a single 2μm vesicle was found. |
'''Preparations''' <br> | '''Preparations''' <br> | ||
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| - | Protocols can be found at [ | + | Protocols can be found at [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Notebook/General_Protocols#Maxiprep | Maxiprep] in the general protocols page |
==Transformation of Biobricks== | ==Transformation of Biobricks== | ||
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**pSB1A3 [pSB1A3] (re-clone)</font> | **pSB1A3 [pSB1A3] (re-clone)</font> | ||
| - | Protocols can be found at [ | + | Protocols can be found at [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Notebook/General_Protocols#Electropotion | Electroporation] in the general protocols page |
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Latest revision as of 15:27, 26 October 2007
10 September 2007
Vesicles
Results
Sample 8, produced on 04.09.2007 was looked at again under the fluorescence microscope, but this time only a single 2μm vesicle was found.
Preparations
Three POPC/dodecane 10ml suspensions were prepared.
Cloning of Biobricks (for Maxiprep)
- Placed 100 ml of LB in a 500 ml conical flask
- Innoculated 1 ml of culture into LB media in flask
- Incubated overnight at 37 °C
- 25. BBa_J37032 [pLux-GFP]
- 13. BBa_B0015 [ ]
Protocols can be found at [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Notebook/General_Protocols#Maxiprep | Maxiprep] in the general protocols page
Transformation of Biobricks
- 1 biobrick was transformed into XL1-Blue
- Cells were grown for half an hour and plated
- Plates were incubated at 37°C overnight
- Transformed in XL1-Blue
- pSB1A3 [pSB1A3] (re-clone)
Protocols can be found at [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Notebook/General_Protocols#Electropotion | Electroporation] in the general protocols page
