Imperial/Wet Lab/Lab Notebook/2007-10-02
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==Test purified luxR== | ==Test purified luxR== | ||
*'''Aim''': The aim of this experiment was to test if the purified luxR works and if the construct 2 works | *'''Aim''': The aim of this experiment was to test if the purified luxR works and if the construct 2 works | ||
| - | *To test the purified luxR we used these [[ | + | *To test the purified luxR we used these [[Imperial/Wet_Lab/Protocols/Prot1.7|Protocol]] |
| - | *The results of this experiment can be found [[ | + | *The results of this experiment can be found [[Imperial/Wet_Lab/Results/ID1.7|Results]] |
*The purified LuxR appeared to be functional in vitro, however there were stability issues that reduce the synthesis of GFPmut3b when pLux-GFPmut3b is used with purified LuxR. | *The purified LuxR appeared to be functional in vitro, however there were stability issues that reduce the synthesis of GFPmut3b when pLux-GFPmut3b is used with purified LuxR. | ||
*For the characterisation of the infector detector system the pTet-LuxR-pLux-GFPmut3b (construct 1) will be used. | *For the characterisation of the infector detector system the pTet-LuxR-pLux-GFPmut3b (construct 1) will be used. | ||
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==Test pT7 in-vivo== | ==Test pT7 in-vivo== | ||
*'''Aim''': The aim of the test was to verify that pT7-GFP expresses in-vivo | *'''Aim''': The aim of the test was to verify that pT7-GFP expresses in-vivo | ||
| - | *The test was carried out using these protocols | + | *The test was carried out using these protocols [[Imperial/Wet_Lab/Protocols/Prot1.9|Protocol]] |
| - | *The results can be found here | + | *The results can be found here [[Imperial/Wet_Lab/Results/Res1.9|Results]] |
Latest revision as of 20:51, 26 October 2007
2 October 2007
Test purified luxR
- Aim: The aim of this experiment was to test if the purified luxR works and if the construct 2 works
- To test the purified luxR we used these Protocol
- The results of this experiment can be found Results
- The purified LuxR appeared to be functional in vitro, however there were stability issues that reduce the synthesis of GFPmut3b when pLux-GFPmut3b is used with purified LuxR.
- For the characterisation of the infector detector system the pTet-LuxR-pLux-GFPmut3b (construct 1) will be used.
Test pT7 in-vivo
- Aim: The aim of the test was to verify that pT7-GFP expresses in-vivo
- The test was carried out using these protocols Protocol
- The results can be found here Results
