Imperial/Wet Lab/Lab Notebook/2007-08-10
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< Imperial | Wet Lab | Lab Notebook(Difference between revisions)
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#Minipreped 2x 14 Biobricks | #Minipreped 2x 14 Biobricks | ||
| - | Protocols can be found at [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Notebook/General_Protocols#Miniprep | + | Protocols can be found at [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Notebook/General_Protocols#Miniprep Miniprep] in the general protocols page |
==Agarose Gel Electrophoresis of Biobricks== | ==Agarose Gel Electrophoresis of Biobricks== | ||
#Checked 2x 14 Biobricks on 1% agarose gel | #Checked 2x 14 Biobricks on 1% agarose gel | ||
| - | Protocols can be found at [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Notebook/General_Protocols#Electrophoresis | + | Protocols can be found at [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Notebook/General_Protocols#Electrophoresis Electrophoresis] in the general protocols page |
[[Image:IC07_Gel_1.JPG|350px]] | [[Image:IC07_Gel_1.JPG|350px]] | ||
Latest revision as of 19:36, 26 October 2007
10 August 2007
Preparation of Stock Solutions
- 1x 500 ml of 2M Tris-acetate pH 8.2
- 1x 500 ml of Buffer A for preparation of S30/S12 cell extract
- 1x 500 ml of Buffer B for preparation of S30/S12 cell extract
- 1x 50 ml of 2mM amino acids
Miniprep of Biobricks
- Minipreped 2x 14 Biobricks
Protocols can be found at [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Notebook/General_Protocols#Miniprep Miniprep] in the general protocols page
Agarose Gel Electrophoresis of Biobricks
- Checked 2x 14 Biobricks on 1% agarose gel
Protocols can be found at [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Notebook/General_Protocols#Electrophoresis Electrophoresis] in the general protocols page
