Imperial/Wet Lab/Lab Notebook/2007-10-17
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==Temperature step test for pTet-GFPmut3b== | ==Temperature step test for pTet-GFPmut3b== | ||
*The '''aim''' of this test was to determine the response of the pTet-GFPmut3b construct to a step change in temperature. | *The '''aim''' of this test was to determine the response of the pTet-GFPmut3b construct to a step change in temperature. | ||
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*The sampling time was every hour | *The sampling time was every hour | ||
*The [[Imperial/Wet_Lab/Results/CBD2.3|Results]] show that the step changes in temperature affect the fluorescence produced by the pTet-GFPmut3b construct | *The [[Imperial/Wet_Lab/Results/CBD2.3|Results]] show that the step changes in temperature affect the fluorescence produced by the pTet-GFPmut3b construct | ||
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Latest revision as of 18:57, 26 October 2007
Temperature step test for pTet-GFPmut3b
- The aim of this test was to determine the response of the pTet-GFPmut3b construct to a step change in temperature.
- For complete materials and methods see the Protocol
- The construct was tested at two temperature steps:
- From 4°C (for 3 hours) to 20°C (3 hours)
- From 20°C (for 3 hours) to 4°C (3 hours)
- The sampling time was every hour
- The Results show that the step changes in temperature affect the fluorescence produced by the pTet-GFPmut3b construct
