Imperial/Wet Lab/Lab Notebook/2007-08-09

From 2007.igem.org

< Imperial | Wet Lab | Lab Notebook(Difference between revisions)
(9 August 2007)
(9 August 2007)
 
Line 4: Line 4:
= 9 August 2007 =
= 9 August 2007 =
-
 
-
 
-
<html></div></html>
 
-
{{Template:IC07labnotebook}}
 
==Cloning of Biobricks==
==Cloning of Biobricks==
#Placed 5 ml of LB in a 15 ml tube
#Placed 5 ml of LB in a 15 ml tube
Line 15: Line 11:
#Grew overnight at 37°C in shaking incubator
#Grew overnight at 37°C in shaking incubator
#*2x 14 Biobricks cloned
#*2x 14 Biobricks cloned
 +
 +
 +
<html></div></html>
 +
{{Template:IC07labnotebook}}

Latest revision as of 13:58, 10 October 2007

9 August 2007

Cloning of Biobricks

  1. Placed 5 ml of LB in a 15 ml tube
  2. Added appropriate antibiotics into the tube
  3. Picked a colony from the fresh overnight plate
  4. Innoculated the colony in the LB media
  5. Grew overnight at 37°C in shaking incubator
    • 2x 14 Biobricks cloned