Imperial/Wet Lab/Lab Notebook/2007-08-10
From 2007.igem.org
(Difference between revisions)
(→Agarose Gel Electrophoresis of Biobricks) |
m |
||
| Line 1: | Line 1: | ||
{{Template:IC07navmenu}} | {{Template:IC07navmenu}} | ||
<html><div id="maincol"></html> | <html><div id="maincol"></html> | ||
| - | + | __NOTOC__ | |
= 10 August 2007 = | = 10 August 2007 = | ||
| Line 14: | Line 14: | ||
#Minipreped 2x 14 Biobricks | #Minipreped 2x 14 Biobricks | ||
| - | Protocols can be found at [ | + | Protocols can be found at [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Notebook/General_Protocols#Miniprep| Miniprep] in the general protocols page |
==Agarose Gel Electrophoresis of Biobricks== | ==Agarose Gel Electrophoresis of Biobricks== | ||
#Checked 2x 14 Biobricks on 1% agarose gel | #Checked 2x 14 Biobricks on 1% agarose gel | ||
| - | Protocols can be found at [ | + | Protocols can be found at [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Notebook/General_Protocols#Electrophoresis|Electrophoresis] in the general protocols page |
[[Image:IC07_Gel_1.JPG|350px]] | [[Image:IC07_Gel_1.JPG|350px]] | ||
Revision as of 12:39, 26 October 2007
10 August 2007
Preparation of Stock Solutions
- 1x 500 ml of 2M Tris-acetate pH 8.2
- 1x 500 ml of Buffer A for preparation of S30/S12 cell extract
- 1x 500 ml of Buffer B for preparation of S30/S12 cell extract
- 1x 50 ml of 2mM amino acids
Miniprep of Biobricks
- Minipreped 2x 14 Biobricks
Protocols can be found at [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Notebook/General_Protocols#Miniprep| Miniprep] in the general protocols page
Agarose Gel Electrophoresis of Biobricks
- Checked 2x 14 Biobricks on 1% agarose gel
Protocols can be found at [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Notebook/General_Protocols#Electrophoresis|Electrophoresis] in the general protocols page
