Imperial/Wet Lab/Lab Notebook/2007-10-01

From 2007.igem.org

< Imperial | Wet Lab | Lab Notebook(Difference between revisions)
m (Evaporation experiments)
 
Line 9: Line 9:
==Evaporation experiments==
==Evaporation experiments==
-
*'''Aim''': The aim of the experiments carried out today was to figure out a method to avoid evaporation of samples.
+
*'''Aim''': The aim of the experiments carried out today was to figure out a method to avoid evaporation of samples. See [[Imperial/Wet_Lab/Protocols/CBD2.1|protocols]] for materials and methods.
*We monitered evaporation of samples in:
*We monitered evaporation of samples in:
**PCR tubes
**PCR tubes
Line 15: Line 15:
**96 well plate with multiple layers of tape
**96 well plate with multiple layers of tape
**96 well plate with single tape and pipetting
**96 well plate with single tape and pipetting
-
*We found out that none of these methods effeciently stop evaporation (more explainantion + link to results!!)
+
*We found out that none of these methods effeciently stop evaporation and pipetting affected the reaction. See [[Imperial/Wet_Lab/Results/CBD2.1|results]] for more details.
<html></div></html>
<html></div></html>
{{Template:IC07labnotebook}}
{{Template:IC07labnotebook}}

Latest revision as of 20:51, 26 October 2007

1 October 2007

Evaporation experiments

  • Aim: The aim of the experiments carried out today was to figure out a method to avoid evaporation of samples. See protocols for materials and methods.
  • We monitered evaporation of samples in:
    • PCR tubes
    • 96 well plate with single tape layer
    • 96 well plate with multiple layers of tape
    • 96 well plate with single tape and pipetting
  • We found out that none of these methods effeciently stop evaporation and pipetting affected the reaction. See results for more details.