Imperial/Wet Lab/Lab Notebook/2007-08-10
From 2007.igem.org
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| + | ==Preparation of Stock Solutions== | ||
| + | *1x 500 ml of 2M Tris-acetate pH 8.2 | ||
| + | *1x 500 ml of Buffer A for preparation of S30/S12 cell extract | ||
| + | *1x 500 ml of Buffer B for preparation of S30/S12 cell extract | ||
| + | *1x 50 ml of 2mM amino acids | ||
| + | |||
| + | ==Miniprep of Biobricks== | ||
| + | #Minipreped 2x 14 Biobricks | ||
| + | |||
| + | Protocols can be found at [[IGEM:IMPERIAL/2007/Notebook/General_Protocols#Miniprep|Miniprep]] in the general protocols page | ||
| + | |||
| + | ==Agarose Gel Electrophoresis of Biobricks== | ||
| + | #Checked 2x 14 Biobricks on 1% agarose gel | ||
| + | |||
| + | Protocols can be found at [[IGEM:IMPERIAL/2007/Notebook/General_Protocols#Electrophoresis|Electrophoresis]] in the general protocols page | ||
| + | |||
| + | [[Image:ICGEMS_Gel_1.bmp|350px]][[Image:ICGEMS_Gel_3.bmp|380px]][[Image:ICGEMS_Gel_2.bmp|380px]] | ||
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Revision as of 13:59, 10 October 2007
Contents |
10 August 2007
Preparation of Stock Solutions
- 1x 500 ml of 2M Tris-acetate pH 8.2
- 1x 500 ml of Buffer A for preparation of S30/S12 cell extract
- 1x 500 ml of Buffer B for preparation of S30/S12 cell extract
- 1x 50 ml of 2mM amino acids
Miniprep of Biobricks
- Minipreped 2x 14 Biobricks
Protocols can be found at Miniprep in the general protocols page
Agarose Gel Electrophoresis of Biobricks
- Checked 2x 14 Biobricks on 1% agarose gel
Protocols can be found at Electrophoresis in the general protocols page
