Imperial/Wet Lab/Lab Notebook/2007-10-05
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==pTet-LuxR-pLux-GFP construct in-vivo== | ==pTet-LuxR-pLux-GFP construct in-vivo== | ||
*'''Aim''': The aim of the test was to check if construct 1 of infector detector works in-vivo | *'''Aim''': The aim of the test was to check if construct 1 of infector detector works in-vivo | ||
| - | *The protocols for the experiment can be found [[ | + | *The protocols for the experiment can be found in the [[Imperial/Wet_Lab/Protocols/ID1.1|Protocols page]] *The [[Imperial/Wet_Lab/Results/ID1.1|results]] of the test showed that pTet-LuxR-pLux-GFPmut3b is working in vivo and GFPmut3b produced increases with increasing [AHL] |
| - | *The [[ | + | |
==pTet-LuxR-pLux-GFP construct in-vitro== | ==pTet-LuxR-pLux-GFP construct in-vitro== | ||
*'''Aim''': The aim of the test was to check if construct 1 of infector detector works in-vitro, for a number of concentrations of AHL | *'''Aim''': The aim of the test was to check if construct 1 of infector detector works in-vitro, for a number of concentrations of AHL | ||
| - | |||
*The [[Media:IC 2007 PLux in-vitro 05-10 25oC.xls|results]] of the test seemed very variable and didn't seemed to comply with the tests that were carried out before. There was a lot of variability between the samples and 4nM of AHL seemed to have fluorescence output greater than 50nM. | *The [[Media:IC 2007 PLux in-vitro 05-10 25oC.xls|results]] of the test seemed very variable and didn't seemed to comply with the tests that were carried out before. There was a lot of variability between the samples and 4nM of AHL seemed to have fluorescence output greater than 50nM. | ||
*It was decided to carry out the experiment again. The reasons for the inaccuracies in the data could have been: | *It was decided to carry out the experiment again. The reasons for the inaccuracies in the data could have been: | ||
Revision as of 18:47, 26 October 2007
5 October 2007
pTet-LuxR-pLux-GFP construct in-vivo
- Aim: The aim of the test was to check if construct 1 of infector detector works in-vivo
- The protocols for the experiment can be found in the Protocols page *The results of the test showed that pTet-LuxR-pLux-GFPmut3b is working in vivo and GFPmut3b produced increases with increasing [AHL]
pTet-LuxR-pLux-GFP construct in-vitro
- Aim: The aim of the test was to check if construct 1 of infector detector works in-vitro, for a number of concentrations of AHL
- The results of the test seemed very variable and didn't seemed to comply with the tests that were carried out before. There was a lot of variability between the samples and 4nM of AHL seemed to have fluorescence output greater than 50nM.
- It was decided to carry out the experiment again. The reasons for the inaccuracies in the data could have been:
- The reaction was not mixed properly or the right amounts were not pipetted
- The plates had already been used and thus could have affected the reaction
