Imperial/Wet Lab/Lab Notebook/2007-08-08
From 2007.igem.org
< Imperial | Wet Lab | Lab Notebook
8 August 2007
Transformation of Biobricks
- 15 biobricks were transformed into XL1-Blue
- Cells were grown for 1 hour and plated
- Plates were at 37°C overnight
- 1. BBa_E0040 [GFP]
- 2. BBa_I13352 [ptet-GFP]
- 3. BBa_J5528 [pBad-GFP]
- 4. BBa_J24677 [ptet-LuxR-plux-GFP]
- 5. BBa_R0040 [ptet]
- 6. BBa_I0500 [pBad]
- 7. BBa_F2620 [ptet-LuxR-plux]
- 8. BBa_F1610 [luxI]
- 9. BBa_R0010 [lacI]
- 10.BBa_E7010 [RFP]
- 11.BBa_E7104 [T7 GFP]
- 12.BBa_S03550 [araC]
- 13.BBa_B0015 [Terminator]
- 14.BBa_R0051 [pCI]
- 15.BBa_S07037 [pCI-GFP]
Protocols can be found at [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Notebook/General_Protocols#Electroporation|Electroporation]in the general protocols page
- Plate 3 had no colony (possibly due to large plasmid size - difficult to transform)
Preparation of Competent Cells
- BL21(DE3)-Gold was made electrocompetent
Protocols can be found at [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Notebook/General_Protocols#Preparation_of_Competent_Cells|Preparation of Competent Cells]] in the general protocols page
