Imperial/Wet Lab/Lab Notebook/2007-08-08

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8 August 2007

Transformation of Biobricks

  1. 15 biobricks were transformed into XL1-Blue
  2. Cells were grown for 1 hour and plated
  3. Plates were at 37°C overnight

  • 1. BBa_E0040 [GFP]
  • 2. BBa_I13352 [ptet-GFP]
  • 3. BBa_J5528 [pBad-GFP]
  • 4. BBa_J24677 [ptet-LuxR-plux-GFP]
  • 5. BBa_R0040 [ptet]
  • 6. BBa_I0500 [pBad]
  • 7. BBa_F2620 [ptet-LuxR-plux]
  • 8. BBa_F1610 [luxI]
  • 9. BBa_R0010 [lacI]
  • 10.BBa_E7010 [RFP]
  • 11.BBa_E7104 [T7 GFP]
  • 12.BBa_S03550 [araC]
  • 13.BBa_B0015 [Terminator]
  • 14.BBa_R0051 [pCI]
  • 15.BBa_S07037 [pCI-GFP]

Protocols can be found at [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Notebook/General_Protocols#Electroporation|Electroporation]in the general protocols page

  • Plate 3 had no colony (possibly due to large plasmid size - difficult to transform)

Preparation of Competent Cells

  1. BL21(DE3)-Gold was made electrocompetent

Protocols can be found at Preparation of Competent Cells in the general protocols page


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