10 August 2007

Preparation of Stock Solutions

• 1x 500 ml of 2M Tris-acetate pH 8.2
• 1x 500 ml of Buffer A for preparation of S30/S12 cell extract
• 1x 500 ml of Buffer B for preparation of S30/S12 cell extract
• 1x 50 ml of 2mM amino acids

Miniprep of Biobricks

1. Minipreped 2x 14 Biobricks

Protocols can be found at [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Notebook/General_Protocols#Miniprep| Miniprep] in the general protocols page

Agarose Gel Electrophoresis of Biobricks

1. Checked 2x 14 Biobricks on 1% agarose gel

Protocols can be found at [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Notebook/General_Protocols#Electrophoresis|Electrophoresis] in the general protocols page