14 September 2007

Restriction Digest of Biobricks

1. 4 DNA samples were digested by SpeI and PstI
   • Samples 5, 7, 24
2. 1 DNA sample wasdigested by XbaI and PstI
   • Samples 23
3. 4 DNA samples were digested by EcoRI
   • Samples 13, 25, 27b-e, T7, Kirsten's parts
4. 4 μl of DNA was digested by 1 μl of enzymes at 37°C for 1 hour
   Exception: Sample 23 had 6 μl DNA and 2 μl enzymes

Protocols can be found at [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Notebook/General_Protocols#Restriction_Digest | Restriction Digest] in the general protocols page

Agarose Gel Electrophoresis of Biobricks

1. Checked 9 Biobricks and 13 Digests on 1% agarose gel

Conclusions:

• Part 27 is confirmed to be faulty
• T7 maxi-prep was not done properly
• Kirsten's parts were most likely not there...

Protocols can be found at [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Notebook/General_Protocols#Electrophoresis | Electrophoresis] in the general protocols page

Gel Purification of Biobricks

1. Gel was melted and run through column
2. Purified DNA was eluted with ddH₂O

Protocols can be found at [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Notebook/General_Protocols#Gel_Purification | Gel Purification] in the general protocols page