Imperial/Wet Lab/Lab Notebook/2007-09-01

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< Imperial | Wet Lab | Lab Notebook(Difference between revisions)
(1 September 2007)
m (Construction of pT7-GFP)
 
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#Plates were incubated at 37&deg;C overnight
#Plates were incubated at 37&deg;C overnight
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Protocols can be found at [[IGEM:IMPERIAL/2007/Notebook/General_Protocols#Transformation|Transformation]] in the general protocols page
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Protocols can be found at [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Notebook/General_Protocols#S30.2FS12_Cell_Extract#Transformation| Transformation] in the general protocols page
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Latest revision as of 13:00, 26 October 2007

1 September 2007

Construction of pT7-GFP

  1. No colony was found in both the ligation and control plates
  2. Transformation into DH5alpha and plating of the cells were repeated
  3. Plates were incubated at 37°C overnight

Protocols can be found at [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Notebook/General_Protocols#S30.2FS12_Cell_Extract#Transformation| Transformation] in the general protocols page