Imperial/Wet Lab/Lab Notebook/2007-10-04

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==Calibration and degradation for GFP mut3b==
==Calibration and degradation for GFP mut3b==
*'''Aim''': The aim of this test was to get a calibration and degrdation curve for GFP mut3b
*'''Aim''': The aim of this test was to get a calibration and degrdation curve for GFP mut3b
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*The protocols used can be found here??
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*The protocols used can be found here: [[Imperial/Wet_Lab/Protocols/Prot1.3|Calibration Protocol]] and [[Imperial/Wet_Lab/Protocols/Prot1.4|Degradation Protocols]]
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*We got a calibration curve from the experiments, which can be seen here
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*We got a calibration curve from the experiments, which can be seen here [[Imperial/Wet_Lab/Results/Res1.3|Results]]
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*The degradation curve can be seen here??. The results showed that the degradation of GFP mut3b is negligible over a period of ?? hours
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*The degradation curve can be seen here [[Imperial/Wet_Lab/Results/Res1.4|Results]]. The results showed that the half life of GFPmut3b is about 1980 minutes, or approximately 33 hours.
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Latest revision as of 20:52, 26 October 2007

4 October 2007

Calibration and degradation for GFP mut3b

  • Aim: The aim of this test was to get a calibration and degrdation curve for GFP mut3b
  • The protocols used can be found here: Calibration Protocol and Degradation Protocols
  • We got a calibration curve from the experiments, which can be seen here Results
  • The degradation curve can be seen here Results. The results showed that the half life of GFPmut3b is about 1980 minutes, or approximately 33 hours.