Imperial/Wet Lab/Lab Notebook/2007-08-03

From 2007.igem.org

3 August 2007

Preparation of Stock Solutions

  • 2x 1 litre of M9 media
  • 2x 1 litre of LB media
  • 4x 500 ml of LB media in conical flasks

Preparation of Antibiotics Plates

  • 30x LB + Amp plates
  • 30x LB + Kan plates
  • 30x LB + Amp + Kan plates
    • 50mg/ml Amplicillin solution
    • 10mg/ml Kanamycin solution

Preparation of Competent Cells

  1. DH5alpha and XL1Blue were made electrocompetent

Protocols can be found at [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Notebook/General_Protocols#Preparation_of_Competent_Cells|Preparation of Competent Cells] in the general protocols page


Retrieved from "http://2007.igem.org/wiki/index.php/Imperial/Wet_Lab/Lab_Notebook/2007-08-03"