Imperial/Wet Lab/Lab Notebook/2007-09-19


19 September 2007

Purification of LuxR-His

  • Today we innoculated 2 litres of LB medium with E.coli containing the luxR-his plasmid (pMU117) to be grown to an O.D.600 0.5. After this the cells were harvested and frozen overnight.
  • In addition an SDS-PAGE gel was run from a small sample of the soluble proeins from these cells, that had been purified using an affinity column (Nickel column for His tag).
  • The gel was left to destain overnight