Imperial/Wet Lab/Lab Notebook/2007-09-28


28 September 2007

Optimum DNA concentration

  • Aim today was to get the optimum DNA concentration for the pTet-GFP and the pTet-lixR-pLux-GFP construct.
  • The constructs were tested for 0, 1, 2, 4 and 6ug of DNA.
  • The fluorescence over a period of 6 hours was recorded every hour for all the concentrations.
  • It was found that at 25°C pTet-lixR-pLux-GFP behaves as expected, i.e. the optimum output is for a mass between 2 and 4ug of DNA.
  • For pTet-GFP construct, at 37°C, the fluorecence was highest at the end of the day for 6ug of DNA.