Imperial/Wet Lab/Lab Notebook/2007-09-19
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| + | ===Purification of LuxR-His=== | ||
| + | *Today we innoculated 2 litres of LB medium with E.coli containing the luxR-his plasmid (pMU117) to be grown to an O.D.<sub>600</sub> 0.5. After this the cells were harvested and frozen overnight. | ||
| + | *In addition an SDS-PAGE gel was run from a small sample of the soluble proeins from these cells, that had been purified using an affinity column (Nickel column for His tag). | ||
| + | *The gel was left to destain overnight | ||
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Latest revision as of 14:11, 10 October 2007
19 September 2007
Purification of LuxR-His
- Today we innoculated 2 litres of LB medium with E.coli containing the luxR-his plasmid (pMU117) to be grown to an O.D.600 0.5. After this the cells were harvested and frozen overnight.
- In addition an SDS-PAGE gel was run from a small sample of the soluble proeins from these cells, that had been purified using an affinity column (Nickel column for His tag).
- The gel was left to destain overnight
