Imperial/Wet Lab/Lab Notebook/2007-10-10

From 2007.igem.org

(Difference between revisions)
Line 5: Line 5:
 +
==Media test==
 +
*The '''aim''' of this test was to see if the fluorescence of GFP mut3b gets affected by the media it is in.
 +
*We tested the reporter's expression in nuclease free water and LB media and then compared it with the reults got from GFP mut3b calibration curve
 +
*The results can be found [[Media:IC 2007 Mediatest.xls|here]]
 +
*It was found a linear relationship between the fluorescence of GFP mut3b in the three media.
 +
*This meant that LB media could be used to get calibration curve for dsRED express instead of cell extract. But as dsRED express couldn't be cloned, we didn't make a calibration curve for dsRED express
 +
 +
==pT7 test in-vitro==
 +
*The '''aim''' of the test was to see if pT7-GFP mut3b and pT7-dsRED express work in-vitro or not
 +
*The protocols can be found [[IGEM:here|here]]
 +
*The experiment didn't give good results as:
 +
**The incubator had been turned off, thus the samples were not at the right temperature.
 +
**The right filter for dsRED express was not used to begin with
 +
*After using the right filter, it was realised that pT7-dsRED express construct doesn't express
 +
*The results can be found [[IGEM:here|here]]
<html></div></html>
<html></div></html>
{{Template:IC07labnotebook}}
{{Template:IC07labnotebook}}

Revision as of 19:44, 24 October 2007

10 October 2007

Media test

  • The aim of this test was to see if the fluorescence of GFP mut3b gets affected by the media it is in.
  • We tested the reporter's expression in nuclease free water and LB media and then compared it with the reults got from GFP mut3b calibration curve
  • The results can be found here
  • It was found a linear relationship between the fluorescence of GFP mut3b in the three media.
  • This meant that LB media could be used to get calibration curve for dsRED express instead of cell extract. But as dsRED express couldn't be cloned, we didn't make a calibration curve for dsRED express

pT7 test in-vitro

  • The aim of the test was to see if pT7-GFP mut3b and pT7-dsRED express work in-vitro or not
  • The protocols can be found here
  • The experiment didn't give good results as:
    • The incubator had been turned off, thus the samples were not at the right temperature.
    • The right filter for dsRED express was not used to begin with
  • After using the right filter, it was realised that pT7-dsRED express construct doesn't express
  • The results can be found here


Retrieved from "http://2007.igem.org/wiki/index.php/Imperial/Wet_Lab/Lab_Notebook/2007-10-10"