Imperial/Wet Lab/Lab Notebook/2007-10-10
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*The '''aim''' of this test was to see if the fluorescence of GFP mut3b gets affected by the media it is in. | *The '''aim''' of this test was to see if the fluorescence of GFP mut3b gets affected by the media it is in. | ||
*We tested the reporter's expression in nuclease free water and LB media and then compared it with the reults got from GFP mut3b calibration curve | *We tested the reporter's expression in nuclease free water and LB media and then compared it with the reults got from GFP mut3b calibration curve | ||
| - | *The results can be found [[Media | + | *The results can be found [[Media:IC 2007 Mediatest.xls|here]] |
*It was found a linear relationship between the fluorescence of GFP mut3b in the three media. | *It was found a linear relationship between the fluorescence of GFP mut3b in the three media. | ||
*This meant that LB media could be used to get calibration curve for dsRED express instead of cell extract. But as dsRED express couldn't be cloned, we didn't make a calibration curve for dsRED express | *This meant that LB media could be used to get calibration curve for dsRED express instead of cell extract. But as dsRED express couldn't be cloned, we didn't make a calibration curve for dsRED express | ||
Revision as of 18:54, 26 October 2007
10 October 2007
Media test
- The aim of this test was to see if the fluorescence of GFP mut3b gets affected by the media it is in.
- We tested the reporter's expression in nuclease free water and LB media and then compared it with the reults got from GFP mut3b calibration curve
- The results can be found here
- It was found a linear relationship between the fluorescence of GFP mut3b in the three media.
- This meant that LB media could be used to get calibration curve for dsRED express instead of cell extract. But as dsRED express couldn't be cloned, we didn't make a calibration curve for dsRED express
pT7 test in-vitro
- The aim of the test was to see if pT7-GFP mut3b and pT7-dsRED express work in-vitro or not
- The protocols can be found here
- The experiment didn't give good results as:
- The incubator had been turned off, thus the samples were not at the right temperature.
- The right filter for dsRED express was not used to begin with
- After using the right filter, it was realised that pT7-dsRED express construct doesn't express
- The results can be found here
