Imperial/Wet Lab/Lab Notebook/2007-10-10

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==Media test==
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*The '''aim''' of this test was to see if the fluorescence of GFP mut3b gets affected by the media it is in.
 +
*We tested the reporter's expression in nuclease free water and LB media and then compared it with the reults got from GFP mut3b calibration curve
 +
*The results can be found [[Media:IC 2007 Mediatest.xls|here]]
 +
*It was found a linear relationship between the fluorescence of GFP mut3b in the three media.
 +
*This meant that LB media could be used to get calibration curve for dsRED express instead of cell extract. But as dsRED express couldn't be cloned, we didn't make a calibration curve for dsRED express
 +
 +
==pT7 test in-vitro==
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*The '''aim''' of the test was to see if pT7-GFP mut3b and pT7-dsRED express work in-vitro or not
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*The protocols can be found [[Imperial/Wet_Lab/Protocols/Prot1.9|Protocol]]
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*The experiment didn't give good results as:
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**The incubator had been turned off, thus the samples were not at the right temperature.
 +
**The right filter for dsRED express was not used to begin with
 +
*After using the right filter, it was realised that pT7-dsRED express construct doesn't express
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*The results can be found [[Imperial/Wet_Lab/Results/Res1.9|Results]]
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Latest revision as of 18:54, 26 October 2007

10 October 2007

Media test

  • The aim of this test was to see if the fluorescence of GFP mut3b gets affected by the media it is in.
  • We tested the reporter's expression in nuclease free water and LB media and then compared it with the reults got from GFP mut3b calibration curve
  • The results can be found here
  • It was found a linear relationship between the fluorescence of GFP mut3b in the three media.
  • This meant that LB media could be used to get calibration curve for dsRED express instead of cell extract. But as dsRED express couldn't be cloned, we didn't make a calibration curve for dsRED express

pT7 test in-vitro

  • The aim of the test was to see if pT7-GFP mut3b and pT7-dsRED express work in-vitro or not
  • The protocols can be found Protocol
  • The experiment didn't give good results as:
    • The incubator had been turned off, thus the samples were not at the right temperature.
    • The right filter for dsRED express was not used to begin with
  • After using the right filter, it was realised that pT7-dsRED express construct doesn't express
  • The results can be found Results


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