Imperial/Wet Lab/Lab Notebook/2007-08-03
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= 3 August 2007 = | = 3 August 2007 = | ||
| + | ==Preparation of Stock Solutions== | ||
| + | *2x 1 litre of M9 media | ||
| + | *2x 1 litre of LB media | ||
| + | *4x 500 ml of LB media in conical flasks | ||
| + | ==Preparation of Antibiotics Plates== | ||
| + | *30x LB + Amp plates | ||
| + | *30x LB + Kan plates | ||
| + | *30x LB + Amp + Kan plates | ||
| + | **50mg/ml Amplicillin solution | ||
| + | **10mg/ml Kanamycin solution | ||
| + | |||
| + | ==Preparation of Competent Cells== | ||
| + | #DH5alpha and XL1Blue were made electrocompetent | ||
| + | Protocols can be found at [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Notebook/General_Protocols#Preparation_of_Competent_Cells|Preparation of Competent Cells] in the general protocols page | ||
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Latest revision as of 12:35, 26 October 2007
3 August 2007
Preparation of Stock Solutions
- 2x 1 litre of M9 media
- 2x 1 litre of LB media
- 4x 500 ml of LB media in conical flasks
Preparation of Antibiotics Plates
- 30x LB + Amp plates
- 30x LB + Kan plates
- 30x LB + Amp + Kan plates
- 50mg/ml Amplicillin solution
- 10mg/ml Kanamycin solution
Preparation of Competent Cells
- DH5alpha and XL1Blue were made electrocompetent
Protocols can be found at [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Notebook/General_Protocols#Preparation_of_Competent_Cells|Preparation of Competent Cells] in the general protocols page
