Imperial/Wet Lab/Lab Notebook/2007-09-12
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==Expression of GFP in vitro under oil conditions== | ==Expression of GFP in vitro under oil conditions== | ||
| - | Today we tested to see if pTet-GFP can express in vitro under oil conditions. We tested the construct to see if there was expression and correct folding of GFP using 30ul paraffin oil. The results are on the following link [ | + | Today we tested to see if pTet-GFP can express in vitro under oil conditions. We tested the construct to see if there was expression and correct folding of GFP using 30ul paraffin oil. The results are on the following link [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Projects/Experimental_Design/Improve_Methodology/Results3 | results] |
==Evaporation under oil under constructs== | ==Evaporation under oil under constructs== | ||
| - | Today we tested the evaporation under different oils. The results can be found on the following link [ | + | Today we tested the evaporation under different oils. The results can be found on the following link [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Projects/Experimental_Design/Improve_Methodology/Results2] From these results we decided to use 30ul of paraffin oil. |
==Maxiprep of Biobricks== | ==Maxiprep of Biobricks== | ||
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| - | Protocols can be found at [ | + | Protocols can be found at [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Notebook/General_Protocols#Maxiprep | Maxiprep] in the general protocols page |
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Revision as of 15:45, 26 October 2007
Contents |
12 September 2007
Expression of GFP in vitro under oil conditions
Today we tested to see if pTet-GFP can express in vitro under oil conditions. We tested the construct to see if there was expression and correct folding of GFP using 30ul paraffin oil. The results are on the following link [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Projects/Experimental_Design/Improve_Methodology/Results3 | results]
Evaporation under oil under constructs
Today we tested the evaporation under different oils. The results can be found on the following link [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Projects/Experimental_Design/Improve_Methodology/Results2] From these results we decided to use 30ul of paraffin oil.
Maxiprep of Biobricks
- Maxipreped 2 Biobricks
- 13. BBa_B0015 [Stop]
- 25. BBa_J37032 [plux-GFP]
Protocols can be found at [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Notebook/General_Protocols#Maxiprep | Maxiprep] in the general protocols page
