Imperial/Wet Lab/Lab Notebook/2007-09-12
From 2007.igem.org
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| + | ==Expression of GFP in vitro under oil conditions== | ||
| + | Today we tested to see if pTet-GFP can express in vitro under oil conditions. We tested the construct to see if there was expression and correct folding of GFP using 30ul paraffin oil. The results are on the following link [[IGEM:IMPERIAL/2007/Projects/Experimental Design/Improve Methodology/Results3| results]] | ||
| + | ==Evaporation under oil under constructs== | ||
| + | Today we tested the evaporation under different oils. The results can be found on the following link [[IGEM:IMPERIAL/2007/Projects/Experimental_Design/Improve_Methodology/Results2]]. From these results we decided to use 30ul of paraffin oil. | ||
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| + | ==Maxiprep of Biobricks== | ||
| + | #Maxipreped 2 Biobricks | ||
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| + | <font size=-2> | ||
| + | *13. BBa_B0015 [Stop] | ||
| + | *25. BBa_J37032 [plux-GFP] | ||
| + | </font> | ||
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| + | Protocols can be found at [[IGEM:IMPERIAL/2007/Notebook/General_Protocols#Maxiprep|Maxiprep]] in the general protocols page | ||
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{{Template:IC07labnotebook}} | {{Template:IC07labnotebook}} | ||
Revision as of 14:09, 10 October 2007
Contents |
12 September 2007
Expression of GFP in vitro under oil conditions
Today we tested to see if pTet-GFP can express in vitro under oil conditions. We tested the construct to see if there was expression and correct folding of GFP using 30ul paraffin oil. The results are on the following link results
Evaporation under oil under constructs
Today we tested the evaporation under different oils. The results can be found on the following link IGEM:IMPERIAL/2007/Projects/Experimental_Design/Improve_Methodology/Results2. From these results we decided to use 30ul of paraffin oil.
Maxiprep of Biobricks
- Maxipreped 2 Biobricks
- 13. BBa_B0015 [Stop]
- 25. BBa_J37032 [plux-GFP]
Protocols can be found at Maxiprep in the general protocols page
